Transduced cells were cloned in G418 and expanded for analysis of IGF-1 transgene expression and its effect on the cell proliferation.
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- 方法 以脂质体为载体,用携带有报告基因绿色荧光蛋白(Green fluorescent protein,GFP)的重组IGF-1转染体外培养的人RPE细胞系,经G418筛选,通过荧光显微镜观察转染情况;