Tricine-SDS-PAGE showed that HPLC fraction 21 ( HLP-3p21) that had potent antimicrobial activity was of a purified single polypeptide with a apparent molecular weight of 14 kDa.

• 将这三个组分用反向高效液相色谱技术进一步分离纯化，琼脂糖弥散法筛选纯化各组分的抗菌活性，较强抗菌活性的组分进行Tricine-SDS-PAGE电泳初步测定分子量及纯度。