Tumor microvessel, density (MVD) was assessed using the expression of CD31. TUNEL ( TdT-mediated biotinylated-dUTP nick end labling method) was adopted to analysis apoptosis of tumor cells.

• 采用TUNEL法(TdT-mediated biotinylated-dUTP nick end labling method)检测各组的细胞凋亡情况,并计算凋亡指数(apoptosis index,AI)。