Using calcein as an indicator, the effects of electroporation on de exined pollen, ungerminated and germinated intact pollen in Nicotiana tabacum were compared.

• 利用膜不通透的荧光染料钙黄素作为指示剂，比较了烟草脱外壁花粉与完整的未萌发花粉和萌发花粉电激导入的效果。