Using mAb F3 as target, a 12-mer phage peptide library was bio-panned for 3 rounds, and the phages eluted from 3rd panning were characterized by sandwich ELISA, competition ELISA and DNA sequencing.
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- 用夹心ELISA和竞争ELISA鉴定筛选克隆的结合特性;并进一步对阳性克隆进行序列测定和分析.;结果通过3轮生物淘选;能被抗体捕获的噬菌体克隆为21/22