Using recombinant plasmid pET28a(+)-GNLY/BL21(DE3)pLysS expression systems, the GNLY protein with biologic activity could be produced, and the strategy is available to prepare human GNLY in large quantity by gene engineering technique.

• 用含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS表达系统,可得到具有生物活性的重组颗粒溶素,为大批量生产提供了条件。