Vestibular ganglion cells were isolated with pipetting after enzymatic treatment for 30 min and loaded with calcium sensitive dye fura-2. Morphology of the VGC and intracellular calcium changes were monitored with digital imaging microscopy.

• 经酶消化后用机械法分离成单细胞,用钙离子敏感的荧光染料Fura-2染色,于数字荧光显微镜下观察。