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- 结果 :在U937中建立四环素调控的WT1基因表达系统。Then the cell line was infected with retrovirus TRE-WT1. Results:A U937 cell line expressing WT1 regulated by the RevTet-On regulatory system was established.
- 方法：采用逆转录聚合酶链反应(RT-PCR)方法测定53例急性白血病患者、15例慢性粒细胞白血病、21名正常人的WT1基因表达。Methods: Expression of WT1 gene was detected in 53 actue leukemia (AL) and 15 chronic myeloid leukemia (CML) patients, 21 healthy subjects by RT-PCR.
- RTPCR检测出EWSWT1融合性mRNA,测序结果显示由EWS基因的7号外显子与WT1基因的8号外显子融合所产生,融合性基因含有KTS序列。Subsequent DNA sequencing showed that the gene fusion involved exon 7 of EWS and exon 8 of WT1 genes. The fusion gene contained KTS sequence.
- 此外,通过采用HAT区缺失突变的p300表达质粒的转染实验,我们证明了p300/CBP的组蛋白乙酰转移酶活性在WT1基因转录调控过程中发挥重要作用。In addition, by using the HAT domain-deleted p300 mutant, weshowed that the histone acetyltransferase activity of p300/CBP was important in regulation ofWT1 gene expression.
- WT1WT1 gene
- 基因代代相传。Genes are transmitted from one generation to another.
- WT1多肽WT1 ploy peptides
- 基因外显率penetrance of gene
- WT1肌病毒WT 1 myovirus
- 分离的基因型a segregant genotype.
- WT1蛋白质类WT1 proteins
- WT1多肽抗原WTI polypeptide
- 基因的区分繁殖differential perpetuation
- Ｃ基因C gene
- 肾母细胞肿瘤抑制因子（WT1）Wilm's tumor suppressor(WT1 )
- Ｄ基因D gene
- Ｊ基因J gene
- Ｖ基因V gene
- Ｉｒ基因Ir gene
- 免疫遗传学免疫学中研究免疫反应的细胞和基因基础的分支The branch of immunology that deals with the molecular and genetic bases of the immune response.
