We cloned the dok1 and dok5 full-length cDNA and expressed their PTB domains, and then the recombinant proteins were purified by affinity, anion exchange and gel filtration chromatography.

• 我们克隆表达了dok1和dok5蛋白，并表达纯化了dok1和dok5的PTB结构域。 经Biacore实验发现，dok1 PTB结构域可特异识别来自Ret的磷酸肽，但不与Shc和Irs1 PTB识别的TrkA和IL-4R磷酸肽结合；