Whereas CFE could be kept for more than 95% if the mega-clone was isolated alone from other KSC daughter cells and disaggregated,replated to form further clones.

• 可见在体外培养条件下KSC克隆细胞逐渐分化，终末分化细胞逐渐增多，总的克隆形成率逐渐降低，但仍保留相当比例的克隆形成细胞。