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- The affinity chromatography can be used in the purification of Fab. 我们可以经过亲和层析 ,纯化基因工程抗体Fab片段
- The fusion protein was purified by GSTrap FF affinity chromatography. GSTrap FF亲和层析柱纯化融合蛋白;
- The purified 6A5 from ascites was about 1.1 mg per ml ascites with affinity chromatography method. 经免疫亲和层析法纯化;腹水中抗体蛋白的得率为1.;1 mg/ml。
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式,溶解和折叠后,以GST亲合层析色谱纯化。
- Made sheep anti?human IgG Fab Sepharose 4B affinity chromatography, purified the souble Fab fragment. 制备羊抗人IgGFab抗血清、建立抗人IgGFab抗体-Sepharose4B亲和层析柱,纯化Fab片段。
- The expressed product issoluble, MW 18kD, which was purified by Ni+ metal chelate affinity chromatography. 表达产物为可溶性蛋白,分子量约为18kD,通过Ni~+离子亲和层析一次性纯化。
- The supernant flows through Ni-NTA gel for affinity chromatography, and thepurity is over 91.5%. 上清经Ni-NTA凝胶亲和层析;纯度达到91.;5%25以上。
- Goat anti human IgG Fab antibody affinity chromatography column was prepared and the Fab supernant was purified. 制备羊抗人IgGFab抗体亲和层析柱、纯化Fab片段。
- Conclusion The method of the monoclonal antibody affinity chromatography is a practicable production procedure. 结论用单克隆抗体亲和色谱法纯化降纤酶是切实可行的。
- Lectins in haemolymph of Sarcophaga peregrina larvae and pupa were purified by affinity chromatography. 用亲和层析法纯化了棕尾别麻蝇幼虫和蛹血淋巴凝集素。
- Objective To purify defibrase from snake venom of Agkistrodon acutus by affinity chromatography. 目的用亲和层析法从尖吻蝮蛇蛇毒中分离纯化降纤酶。
- I.Dissociation of the complex and recoery of the natie haptoglobin in an affinity chromatography system. 请有资源的战友帮忙查询一篇文章,The hemoglobin-haptoglobin bond.
- Affinity chromatography used to play important rule in the separation and purification of biomacromolecule. 亲和色谱多用于生物大分子的分离与纯化。
- Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography. 而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。
- Affinity chromatography was carried out based on the bioaffinity ligand selected. 结果筛选出两个可以特异结合人血清白蛋白的亲和配体。
- A high performance liquid chromatographic method, "hydrophobic interfacial affinity chromatography", to separate commercial Candida rugosa lipase has been established. 建立一种“疏水界面亲和色谱”分离柱状假丝酵母脂肪酶同工酶的高效液相色谱新方法。
- The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography. 经表达和纯化获得了纯度达90%25以上的融合蛋白;
- ADP affinity chromatography was used to purify HSP70 peptide complexes from mouse liver or tumor of mouse for studies in vitro . 采用二磷酸腺苷 (ADP)琼脂糖亲和层析的方法 ,从小鼠肝脏或肿瘤组织中纯化热休克蛋白 (HSP) 70多肽复合物。
- Several kinds of IgG from different animal sera, orascites were purified by Hitrap protein G affinity chromatography, and their purities were compared. 比较 Hitrap G蛋白 Sepharose亲和色谱系统对不同动物血清或腹水的纯化效果 ,为抗体纯化提供依据。
- Affinity chromatography with one of these immunomodulatory peptides had identified heme oxygenase-1 (HO-1) as a potential receptor for these agents. 亲和层析法研究表明,HLA衍生肽的作用机制可能与血红素氧化酶-1(heme oxygenase -1, HO-1)有关。