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- allele specific amplification(ASA) 等位基因特异性扩增
- Single-tube bi-directional allele specific amplification 单管双向等位基因专一性扩增
- A simple and rapid new method for SNP typing by single-tube bi-directional allele specific amplification 单管双向等位基因专一性扩增的单核苷酸多态分型的新方法
- Application of Single-Tube Bi-Directional Allele Specific Amplification in SNP Genetic Monitoring of Inbred Mice 单管双向等位基因专一性扩增方法在近交系小鼠遗传检测中的应用
- Keywords allogeneic hematopoietic stem cell transplantation;chimerism;single nucleotide polymorphism;real-time PCR;phosphorothioate modified;allele specific amplification; 异基因造血干细胞移植;嵌合体;单核苷酸多态性;实时荧光PCR;硫代化修饰;等位基因特异性扩增;
- allele specific amplification 等位基因特异性扩增
- Addition of formamide to PCR system will efficiently inhibit non-specific amplification, increase specific amplification and sensitivity up to an order of magnitude. 在多聚酶链式反应体系中加入甲酰胺可以有效地抑制非特异性扩增,提高特异性扩增,并使反应的灵敏度提高一个数量级。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
- Methods By RT-PCR,specific amplification products of P2 gene from H2 final product and its parent seed were obtained,then sequenced and blasted to analyze its virulence gene VP1/2A and 2C. 方法通过逆转录-聚合酶链反应,特异扩增甲肝减毒活疫苗H2株成品疫苗及其亲本毒种P2区VP1/2A、2B、2C基因序列,经测序、比对,分析检测毒力基因VP1/2A、2C的变异情况。
- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型，方法简单实用，在法医学个人识别领域具有较高的应用。
- mutant-allele specific amplification 突变等位基因扩增
- allele specific oligonucleotide(ASO) 等位基因特异性寡核苷酸, 等位基因特异的
- allele specific oligonucleotide hybridization 等位基因特异寡核苷酸杂交
- Allele specific polymerase chain reaction 等位基因特异性聚合酶链反应
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。
- He gave us very specific instructions. 他给我们做了非常明确的指示。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定，首次鉴定了我国主栽的一些甜樱桃品种的S基因型，并发现了两个新的S基因型S1S6、S4S9，将自交不亲和的组群扩展到21个。
- There is a specific tool for each job. 各工种配备特定的工具。
- But that all depend on the specific circumstances. 不过，那要看具体情况而定。
- Some of them require further amplification. 其中有些内容需进一步详细阐明。
