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Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型，方法简单实用，在法医学个人识别领域具有较高的应用。
We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定，首次鉴定了我国主栽的一些甜樱桃品种的S基因型，并发现了两个新的S基因型S1S6、S4S9，将自交不亲和的组群扩展到21个。
Keywords Plasmodium vivax;circumsporozoite protein（CSP）;nest allele specific PCR;different size DNA band; 间日疟原虫;环子孢子蛋白基因;套式等位特异PCR;多样性DNA片段;
Keywords Forensic biological evidence;Y chromosome;Biallelic marker;Tragment length discrepant allele specific PCR;Haplogroup; 法医物证学;Y染色体;双等位基因标记;片段长度差异等位基因特异性PCR;单体群;
allele specific PCR 等位基因特异性多聚酶链反应
allele specific PCR(AS-PCR) 等位特异性PCR, 等位基因特异性扩增技术
Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
We have achieved lots of exciting achivements in experting Solar power; different categories of clothing and textile stocks;and the rubber production,such as PCR,TPR,OTR etc. 在各类男、女、儿童四季服装和各类库存纺织品；橡胶制品轿车胎.;载重胎
allele specific amplification(ASA) 等位基因特异性扩增
allele specific oligonucleotide(ASO) 等位基因特异性寡核苷酸, 等位基因特异的
Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection. 利用MSP检测白血病细胞株在5-Aza CdR处理前后、MG88的转染前后PRA、PRB启动子CpG岛甲基化状态。
allele specific oligonucleotide hybridization 等位基因特异寡核苷酸杂交
If food was cultured for 25 hours for proliferation in listeria enrichment broth, then the culture was chemically extracted, the purified bacteria after heat lysis could react as PCR templates and the inhibitory effects could be greatly reduced. 经过25小时的增菌培养，对培养物进行化学抽提、纯化的菌体经加热裂解后直接用作pcr反应模板，可使抑制作用大大降低。