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- XL2 was purified by three steps including breaking cell wall,ammonium sulfate precipitation,and gel filtration chromatography(GFC). 方法探讨了不同破壁方法和硫酸铵盐析提取粗酶的条件。
- A phospholipase C (PLC) was successfully purified by ammonium sulfate precipitation followed by a application of ion-exchange chromatography (DEAE-Sepharose Fast Flow) and gel filtration (Sephadex G-100 chromatography). 先后采用(NH4)2SO4分级盐析、DEAE-Sepharose Fast Flow阴离子交换层析、Sephadex G-100凝胶过滤、再次DEAE-Sepharose Fast Flow阴离子交换层析等方法从哈维氏弧菌SF-1菌株的上清液中分离纯化得到磷脂酶C。
- A trypsin like enzyme(AGP, Mr 14kD) was purified after ammonium sulfate precipitation, Sephadex G 75 gel filtration, Sephadex DEAE A 25 ion exchange chromatography and SBBI Sepharose 4B affinity chromatography. 肠液经硫铵沉淀,SephadexG?75凝胶过滤,SephadexDEAEA?25离子交换和SBBI?Sepharose4B亲和层析,分离纯化出分子量约为14kD的巨尾阿丽蝇蛋白酶。
- This paper reports the purification of CCO from bovine heart with ammonium sulfate precipitation, phneyl - Sepharose CL-4B hydrophobic chromatographic and cytochrome C-Sepharose 4B affinity chromatographic methods. 本文应用硫酸铵分级沉淀、Phenyl-Sepharose CL-4B 疏水层析和 Cytocrome C-Sepharose 4B亲和层析的方法,从牛心肌中分离纯化了细胞色素C氧化酶。
- In this paper, based on the general physical and chemical character of MTG, several satisfied methods was decided after studied on using high centrifugation, ammonium sulfate precipitation, alcohol precipitation, acetone precipitation to separation MTG. 本文根据微生物谷胺酰胺转胺酶的基本理化性质,先后采用了高速离心、硫酸铵盐析沉淀、乙醇沉淀、丙酮沉淀等方法,对微生物谷胺酰胺转胺酶的初步提纯工艺进行了研究讨论,确定了该酶不同用途的初提方法,并为该酶的进一步纯化做好准备。
- ammonium sulfate precipitation method 硫酸铵沉淀法
- ammonium sulfate precipitation procedure 硫酸铵沉淀法
- Specific acti vity of raw extracts of Allium fistulosumL. is 15.42 U/mg, which turn to 26.46U/mg after ammonium sulfate precipition. 大葱粗提液比活力为15.;42U/mg;经(NH_4)_2SO_4盐析后比活力为26
- Separation and Purification of Nattokinase Produced by Bacillus Subtilis with Ammonium Sulfate Precipitation and Chromatography 硫酸铵沉淀和层析法分离纯化纳豆激酶的研究
- ammonium sulfate precipitation 硫酸铵沉淀
- Precipitation of metals under hydrothermal conditions has the advantage of a compact plant but the disadvantage of producing ammonium sulfate as a coproduct. 金属在湿法加热条件下沉淀有利的是设备密实,不利的是产出了副产品硫酸铵;
- Methods:First,purifying the enzyme by precipitation with ammonium sulfate,then through CM Sepharose Fast Flow chromatography.The purity and the molecular weight was detected by the SDS-PAGE. 方法:先用硫酸铵沉淀,然后通过羧甲基琼脂糖快速胶柱层析后进行分离纯化,并用SDS-PAGE测定纯度、相对分子质量,及其对人血凝块的水解活性。
- Annual sales: steel, Crude Benzol, Coke, tar, ammonium sulfate, and so on. 常年销售:钢材,粗苯,焦碳,焦油,硫酸铵等。
- Ammonium sulfate deposition were used to get kidney bean phytohemagglutinin. 用硫酸铵沉淀法得到芸豆植物凝集素。
- TWO STEPS METHOD FOR THE PREPARATION OF POTASSIUM SULFATE BY AMMONIUM SULFATE AND POTASSIUM CHLORIDE. 硫酸铵氯化钾两次反应法生产硫酸钾。
- Why isn't ammonium sulfate included in the dialysis buffer? Why do you use the buffer solution instead of water? 为什么此步中透析缓冲液中不加硫酸铵?为什么使用缓冲液而不用水?
- The effect of ultrasonic on the preparation of magne sia from magnesite,ammonium sulfate and salvolatile was studied. 研究了超声波在以菱镁矿、硫酸铵和碳酸铵为原料,制备活性氧化镁过程中的作用。
- Furthermore, the influence of ammonium sulfate on the separation of amino acids mixture was investigated. 还考察了在混合物中含有硫酸按时的情况。
- Oxymyoglobin was partly oxidated into the met-myoglobin in the process of the ammonium sulfate salting-out. 在盐析过程中,部分氧合肌红蛋白氧化成为高铁肌红蛋白。
- In this paper, a procedure of isolation and purification of human serum high density lipoprotein (HDL) by dextran sulfate precipitation in combination with gel filtration chromatography was reported. 本文用DS和Ca~(++)沉淀法结合凝胶过滤法分离纯化了人血清HDL。