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- Fas antigen expression on BGC-823 cell surface was detected with Fas monoclonal antibody and then analyzed by FCM. 用Fas单克隆抗体检测BGC-823细胞表面Fas的表达;
- Cell cycle and apoptosis rate was analyzed by flow cytometry. Fas antigen expression on BGC-823 cells surface was detected with Fas monoclonal antibody and then analyzed by FCM. 流式细胞术检测熊果酸作用后BGC-823细胞周期的改变和药物作用后细胞的凋亡率,以及BGC-823细胞表面Fas表达的改变;
- Apoptosis could be observed without the expression of FasL when anti Fas antibody was added to RMA cells. 在无FasL表达情况下 ,抗Fas单克隆抗体能诱导RMA细胞凋亡。
- Aim The phage display antibody library was constructed to obtain functional anti Fas Fab. 目的构建噬菌体抗体库,获得具有功能的抗Fas?Fab噬菌体抗体。
- Objective:To investigate the mechanism inducing apptosis of human glomerular mesangial cells (Mcs) by anti Fas antibody. 目的:研究抗Fas抗体对人肾小球系膜细胞的致凋亡作用,探讨Fas?FasL在肾脏损伤中的作用。
- Study on the monoclonal antibody to chlorpyrifos. 抗毒死蜱单克隆抗体的研制。
- Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application. 结论制备的可溶性抗Fas?Fab抗体具有抗Fas抗体的特异性,为进一步的研究奠定了基础。
- Methods Anti human bladder carcinoma cell line BIU 87 monoclonal antibody BDI 1 was labelled with technetium 99m by direct mercaptoethanol reducing method. 方法采用抗人膀胱癌单克隆抗体BDI-1通过2-巯基乙醇直接还原法标记99mTc(99mTc)制备了99mTc-BDI-1。
- After pretreatment of anti ICAM 1 and E selectin monoclonal antibody, serum LDH and LAT levels decreased significantly; KBR and ATP content of liver increased. 抗ICAM 1及E selectin单抗预处理可使急性胆管炎大鼠血浆LDH ,ALT水平明显降低 ,KBR及肝组织ATP含量升高。
- An alternative to the costly monoclonal antibody approach. 可替代昂贵的单克隆抗体分析方法。
- Objective: To express Fab fragments of the anti colonic cancer antibody and to compare the antigen binding characteristics between the expressing product and the authentic monoclonal antibody. 目的:在大肠杆菌中表达抗结肠癌抗体Fab片段,比较表达产物和原杂交瘤单克隆抗体的抗原结合特性。
- Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 结果3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%25左右,Fab抗体柱纯化的Fab抗体的纯度为95%25,但这两种亲和柱的目的蛋白收率都不高,分别为35%25、55%25。
- The results of detection of specific IgG4 in sera of 173 cerebral cysticercosis patients by dip test using gold labeled anti human IgG4 monoclonal antibody(McAb) were reported and compared with the case CT and MR scans. 应用金标抗人IgG4 McAb 浸测试验检测173 例脑囊虫病患者血清中特异IgG4,并与其脑CT、MR影像分型进行对比分析。
- Objective To investigate the relationship between expression level of Fas and anti Fas biological responsiveness. 目的 研究细胞表面Fas 表达水平与抗Fas 治疗生物学效应之间的关系。
- Objective To develop monoclonal antibody Fab fragments using display technique. 目的 利用噬菌体抗体库技术制备抗甲状旁腺激素相关蛋白 (PTHrP)Fab抗体。
- Methods Immunohistochemistry using PCNA monoclonal antibody PC10 cvere studied. 方法用增殖细胞核抗原(PCNA)单克隆抗体PC10免疫组化研究。
- The monoclonal antibodies are aimed at EPF. 本研究制备的EPF单克隆抗体为特异性抗EPF抗体。
- Methods The percentage of apoptotic cells was detected after childhood ALL cells were incubated with Bcl 2 ASON, anti Fas MoAb or with both of them, respectively. 方法小儿急淋白血病细胞分别与Bcl?2反义脱氧寡核苷酸、抗Fas单抗培养,或与两者一起培养,再分别定量检测其凋亡细胞。
- Meanwhile, anti human BPI polycolonal antibodies or monoclonal antibodies increased the binding ability of porcine BPI to endotoxin. 同时抗人BPI多克隆抗体和单克隆抗体可增加猪源BPI的结合内毒素活性。
- The neurites were also Identified by immunochemical staining using monoclonal antibody to neurofilament. 利用免疫化学染色,这些生长的神经轴突可被对抗神经细丝的单株抗体所染色。