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- Expression and Detection of Different Fragments of HIV-1 Envelope Genes in a Bac-to-Bac Baculovirus Insect cell Expression System 不同区段HIV-1 env基因在Bac-to-Bac昆虫细胞杆状病毒表达系统中的表达及检测
- The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Abstract: The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要: 昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Summers M D,Smith G E.A manual of methods for baculovirus vectors and insect cell culture procedures[J].Texas Agricultural Experiment Station Bulletin,1987,1555-1556. 洪华珠彭建新.;一株高水平表达重组蛋白昆虫细胞系的建立[J]
- Objective Bac-to-Bac baculovirus expression system was used to obtain biologically active recombinant human basic fibroblast growth factor(hbFGF) in insect cells. 目的利用Bac-to-Bac杆状病毒表达系统,将人碱性成纤维细胞生长因子(human basic fibroblast growth factor,hbFGF)基因进行表达,并检测重组蛋白的特异性及生物学活性。
- A new chlorate cell system with a DSA anode and an oxygen cathode was set up. 利用氧阴极与DSA阳极组装成一种新型氯酸盐电解槽。
- Keywords hepatitis B virus HBcAg Baculovirus Expression Vector System insect cell; 乙型肝炎病毒;乙型肝炎病毒核心抗原;杆状病毒表达系统;秋粘虫细胞;
- Co-transfection of SF9 insect cells was performed with rpVLgB and Baculovirus linear DNA (BAC-N-Blue DNA). After three times purification, a recombinant baculovirus, designated as rpVL-ILTVgB was obtained. 将rpVLgB转移载体质粒与杆状病毒DNA(Bac-N-Blue DNA)共转Sf9昆虫细胞,经三轮蚀斑纯化,获得的重组病毒命名为rpVL-ILTVgB。
- Keywords ScFv;Diabody;Hantaan virus;Baculovirus;Insect cell; 单链抗体;双链抗体;汉滩病毒;杆状病毒;昆虫细胞;
- Keywords MC1R gene;Cloning;Expression;Baculovirus;Insect cell; 关键词MC1R基因;克隆;表达;杆状病毒;昆虫细胞;
- In the Hep-2 cell system,the ED50 of ACV in inhibiting the hyperplasia of HSV-2 333 strain was 10.85ug/ml. 在Hep-2细胞系统中;ACV对HSV-2333株增殖抑制的ED50为10.;85ug/ml。
- Keywords adiponectin;baculovirus;insect cell;expression; 脂联素;杆状病毒;昆虫细胞;表达;
- Blowers play key roles in the operation of fuel cells system. 鼓风机为燃料电池发电系统中重要零组件。
- J.Larminie and A.Dicks,“Fuel Cell System Explained”, J.Wiley , chapter 1-7, pp. 1-226, 2003. “台湾燃料电池产业发展策略之研究”,台湾经济研究院,民国九十年十一月。
- Electrolytic cell system for ultrasonic electro-oxidation of molybdenite was studied and designed. 进行了辉钼矿超声电氧化新工艺电解槽体系的研究与设计。
- In this paper, a MTT-based semiautomated colormetric assay was used to study the sensitivity difference among eight cultured insect cell lines to Methomyl pesticide. 采用MTT法,研究了灭多威农药对八种昆虫离体细胞系敏感性的差异。
- A conventional cell system can not easily realize macrodiversity, or it can only realize partially macrodiversity. 而传统的蜂窝系统结构不容易实现宏分集,或者只能采用选择式宏分集。
- These indicated that pieces of E. coli and pieces of insect cell that expressed 16L1 proteins could replace purified HPV16L1 proteins as antigen in ELISA test. 表明用表达16L1的大肠埃希菌破碎物和表达16L1的昆虫细胞破碎物可以代替纯化的HPV16L1蛋白作为抗原进行ELISA试验。
- Test results show that the communications protocol and monitoring program designed can meet the control requirements of fuel cell system. 实验结果表明,设计的通信协议和监控程序能够满足燃料电池系统的控制要求。
- P.R.Pathapati , X.Xue and J.Tang, " A new dynamic model for predicting transient phenomena in a PEM fuel cell system", Renewable Energy, 2006. 邱冠铭,”质子交换膜燃料电池之暂态性能分析”,国立成功大学系统及船舶机电研究所,2006。