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- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- blot hybridization technique 吸印杂交技术, 印迹杂交技术
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- To develop a universal PCR-reverse blot hybridization assay, which aime to detect and identify pathogenic yeast rapidly and exactly. 摘要建立PCR结合寡核苷酸探针反向斑点杂交技术,快速检测及鉴定致病性酵母菌。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交(PCR/ASO)技术。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法:用地高辛素标记基因探针,斑点杂交法检测未知和已知病原的肝炎患者75例,正常对照17例。
- Somatic hybridization technique can overcome some difficulties in traditional breeding and shorten breeding period in tobacco. 利用体细胞杂交技术;可以克服传统的有性杂交遇到的诸多育种障碍;加速烟草品种选育进程.
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 为了探究内皮素?1(ET?1)对肾功能的影响和作用方式,采用斑点杂交和原位杂交方法对大鼠缺血60分钟再灌注肾组织ET?1及其受体亚型(ETA、ETB)的基因表达进行了研究。
- Methods: Integrated optical density (IOD) was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. 方法:采用ABC免疫组织化学和原位杂交组织化学技术,结合计算机图像处理,测定其积分光密度(IOD)值。
- Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法:用LE11.;8、MYO和M13探针,经Southern杂交法检测12例儿童急性粒细胞白血病患者的外周血或骨髓细胞的基因重排。
- The new "hybrid technique" is performed in a catheterization laboratory, where the chest is opened briefly, Bacha explained. 这种新的“混合技术”在心导管室进行,在那儿迅速打开胸壁,给双侧肺动脉放置限制带,然后用支架来保持动脉导管开放,使血液从肺动脉流进主动脉。
- Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑点杂交技术和免疫组化染色测定40例正常前列腺(NP)、38例前列腺增生症(BPH)组织中b-FGF的表达情况。
- Capillary electrochromatography(CEC) was considered to be a hybrid technique that combines the feature of both HPLC and Capillary electrophoresis. 毛细管电色谱(CEC)有机地结合了高效液相色谱的多选择性和毛细管电泳的高效性,是一种新型的微柱分离方法。
- Application of Fluorescence Insitu Hybridization Technique for Prenatal Diagnosis of Chromosome Abnormality in Amniotic Cells HUANG Yanyi, SUN Xiaofang, LI Qing, et al. 色体产前诊断具有快速、准确等优点,特别对染色体结构异常与来源不明的标记染色体诊断,具有更高的敏感性和可靠性。
- The hybrid technique is simple, easily realizable, and offer fast convergence.The inverter switching method is Space Vector PWM which makes the lower harmonic losses. 此控制方式的优点为简单、易实现及有较佳的收敛时间,再配合上电压空间向量波宽调变技术,使切换损失达到最小。