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- The new structure allowed the team to generate a detailed model of how the binding between the botulinum neurotoxin component and SNAP-25 that leads to cleavage comes about. 根据之前研究的结果，它们设计出的分子能够抑制可降解神经细胞中的SNAP-25蛋白的这种毒素的部分。
- Human ScFv against botulinum neurotoxin serotype A(BoNTa) was modified by fusing human IgG1 Fc to C terminal of ScFv. ScFv-Fc fusion protein was expressed at high level over 30% of total host cell proteins in E. coli. 以获得的抗A型肉毒毒素单链抗体为模板,进行融合改构,将人IgG1的Fc片段连接到ScFv的C端,在大肠杆菌中实现抗体融合蛋白ScFv-Fc的表达,表达量30%25以上,蛋白以包含体形式存在,经过体外变复性的抗体融合蛋白ScFv-Fc,进行Prote in G Sepharose柱亲和层析纯化,纯度达90%25~95%25。
- A botulinum neurotoxin blocked the exocytosis completely but not the vacuole formation. Cytochalasin D, an actin filament modifier, did not affect the formation of vacuoles but prolonged its time course. 肉毒杆菌神经毒素可以阻断分泌的过程但是并不能阻断空泡的形成,细胞松弛素D不能阻断空泡的形成,但是可以延长空泡的持续时间。
- Advance in Botulinum Neurotoxin Inhibitors 肉毒毒素抑制剂的研究进展
- Keywords Clostridium botulinum;botulinum neurotoxin;B cell epitope; 肉毒梭菌;肉毒神经毒素;B细胞表位;
- Keywords botulinum neurotoxin;neurotransmitter;synaptic vesicle; 肉毒神经毒素;神经递质;突触小泡;
- Keywords Botulinum neurotoxin serotype A;Phage library;Human ScFv;Expression; 型肉毒毒素;噬菌体文库;人源单链抗体;表达;
- Hologram Quantitative Structure-Activity Relationship Study of Botulinum Neurotoxin A Inhibitors A型肉毒毒素抑制剂的分子全息定量构效关系研究
- Screening of Botulinum Neurotoxin type A-binding peptides by phage display peptide library 以表位模拟肽为亲和靶标的肉毒毒素受体结合抑制剂的筛选及鉴定
- Purification of Recombinant Protective Fragment of Botulinum Neurotoxin Serotype A by IEF IEF纯化重组A型肉毒毒素保护性抗原
- Keywords botulinum neurotoxin type A;human ScFv;ex pression;purification;E.coli; 型肉毒毒素;人源单链抗体;表达;纯化;大肠杆菌;
- Keywords botulinum neurotoxin serotype A;protective antigen;modification;expression; 关键词A型肉毒毒素;保护性抗原;基因修饰;高效表达;
- Keywords botulinum neurotoxin;Clostridium botulinum;botulism;toxin warfare agents;bioterrorism;medical protection; 肉毒神经毒素;肉毒梭菌;肉毒中毒;毒素战剂;生物恐怖;医学防护;
- Keywords botulinum neurotoxin A;inhibitor;hologram;quantitative structure-activity relationship; 型肉毒毒素;抑制剂;分子全息;定量构效关系;
- Expression, Purification and Specific Monoclonal Antibody Preparation of Protective Hc Gene of Botulinum Neurotoxin Serotype A A型肉毒毒素Hc基因的原核表达、纯化及单抗的制备
- Botox is the marketing name given to a neurotoxin called botulinum toxin A. 肉毒杆菌是一种名为肉毒杆菌A的神经毒素的市场化名。
- Botulinum neurotoxin serotype A A型肉毒毒素
- Botulinum neurotoxin type B B型肉毒毒素
- botulinum neurotoxin A A型肉毒毒素
- botulinum neurotoxin BoNT 肉毒神经毒素
