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- Objective To screen the pancreatic cancer-related gene by cDNA microarray. 摘要目的应用基因微矩阵芯片筛选胰腺癌的相关基因。
- Quantity of labeled cDNA was mixed and hybridized to the cDNA microarray, which contain 13824 unigene and EST. 取等量两种探针混合后与含有13824个基因的人类全基因表达谱芯片杂交。
- Differences in gene expressions were compared by cDNA microarray in skeletal muscle of type 2 diabetic rats and normal rats. 用基因芯片技术比较2型糖尿病大鼠和正常大鼠骨骼肌基因表达谱的差异。
- ObjectiveTo develop a human glioma-specific cDNA microarray having own intellectual property, and to promote large-scale tumor expression study. 目的:构建人脑胶质瘤特异的基因表达谱芯片,促进大规模胶质瘤表达谱的研究。
- Objective To screen the differentially expressed genes between esophageal carcer and carcer adjacent tissue using cDNA microarray. 目的应用微矩阵表达谱基因芯片研究食管癌的基因变化。
- Methods The expression of mRNA from PBMC of CML patients was compared with that of normal controls using a cDNA microarray . 方法 应用基因表达谱芯片技术,比较CML患者和正常人外周血单个核细胞(PBMC)基因的表达差异。
- Objective To develop a human glioma-specific cDNA microarray having own intellectual property,and to promote large-scale tumor expression study. 目的:构建人脑胶质瘤特异的基因表达谱芯片,促进大规模胶质瘤表达谱的研究。
- Methods cDNA microarray chips containing 588 rat target genes were used to identify gene expression profile in synovitis of CIA rats and normal rats. 方法用含588个基因的大鼠基因表达谱芯片研究胶原诱导性关节炎及正常大鼠滑膜基因表达谱。
- Normal liver cell line HL-7702 was used as control,and 8 liver samples from 2 patients without CGS and 6 patients with CGS were studied by cDNA microarray containing 10 000 genes. 正常肝细胞株(HL-7702)为共同参照,用含10 000个cDNA克隆的芯片对8份肝脏标本样品(包括6例胆囊结石和2例无胆囊结石对照)进行基因表达谱对比分析;
- In this study, we first applied cDNA microarray technique to analyze the differentially expressed genes between 2 pairs of AAA and normal aortic tissues. 本研究首先应用基因芯片技术对2对AAA和正常主动脉组织进行了基因差异表达分析。
- Methods:The cDNA microarray was used to detect the gene expression of 3 infants with anorectal malformation and normal rectal tissues of 3 infants without the malformation. 方法:应用基因表达谱芯片技术对3例先天性肛门直肠畸形患儿的直肠末端组织和3例正常直肠末端组织的基因表达进行检测。
- Recently, using cDNA microarray technology, DLBCL can be divided into prognostically significant subgroups with germinal center B-cell-like (GCB), activated B-cell-like (ABC), or type 3 gene expression profiles. 2000年以来国外利用cDNA微阵列技术发现可以将DLBCL分为三型:(1)生发中心样(germinal center B-cell-like, GCB);
- Objective To screen acute rejection related genes of allogeneic cardiac transplantation by cDNA microarray technique and study the mechanism of acute rejection of allogen eic cardiac transplantation in rats. 目的运用基因芯片技术分析同种异基因大鼠心脏移植后急性排斥反应的基因表达谱。
- Objective To survey carboxypeptidase E(CPE) mRNA expression by cDNA microarray and real-time PCR in the liver of patients with cholesterol gallstone(CGS),and to reveal the role of CPE in the formation of CGS. 目的:利用基因芯片和实时定量聚合酶链反应(real-time PCR)分析胆囊胆固醇结石病人肝脏中羧肽酶E(carboxypeptidase E,CPE)的mRNA表达,探讨CPE与胆囊胆固醇结石形成可能相关的分子机制。
- Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated. cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中,筛选到了152个基因和EST 序列在去神经10天海马差异表达,表达变化在1.;5-2
- In 2004, using the cDNA microarray results as a golden standard, Hans et al found that the expression of CD10, BCL-6 and MUM1 can be combined to divide cases of DLBCL into GCB and non-GCB subgroups with an outcome closely related to prognosis. 2004年Hans等人运用cDNA微阵列作为参照,采用三种基因蛋白产物(CD10,BCL-6,MUM1)免疫组化标记方法,据免疫表型将DLBCL简单地分成预后有显著差异的GCB与non-GCB两类分子亚型,同样提示前者预后较好。
- Methods Analyzing different concentrations of testosterone stimulate tumor cell proliferation and expression of ARGs under testosterone treatment in LNCaP by using cDNA microarray. 方法检测不同浓度的睾酮对雄激素依赖性前列腺癌细胞LNCaP增殖的影响,采用基因芯片确定LNCaP细胞中受睾酮调控的基因。
- Results A cDNA microarray analysis consisting of 4 096 human genes revealed significant changes in the expression of 369 genes, with 228 genes being up-regulated and 141 being down-regulated. 结果基因芯片包括4096条,基因表达变化显著差异的有369条,228条上调,141条下调。
- A new method labelling cDNA microarray probe 一种标记cDNA芯片探针的新方法
- Keywords Hepatitis C virus;Splice;cDNA microarray; 丙型肝炎病毒;剪切体;基因芯片;
