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- CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 云南省烟草花叶病毒和黄瓜花叶病毒外壳蛋白基因克隆及序列分析。
- We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. 艾滋病相关病毒(ARV-2)是目前应用最广的艾滋病毒分离株之一.
- Methods:APAF-1 cDNA types in leukemic cell lines K562 and CEM/VLB100 were studied by cDNA cloning strategy. 方法:应用RT-PCR结合测序方法研究白血病细胞系K562和CEM/VLB100APAF-1cDNA类型;
- A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。
- Objective To construct the cDNA subclones spanning the entire genome of dengue 2 virus NGC strain for further construction of full-length infectious viral cDNA clone. 目的构建登革2型病毒NGC株基因组全序列的亚cDNA克隆,为进一步构建全长感染性cDNA克隆奠定基础。
- A cDNA clone encoding a pheromone binding protein 3 from antenna of Helicoverpa assulta (named HassPBP3) was isolated by reverse transcription polymerase chain reaction (RT-PCR). 利用RT-PCR技术从烟实夜蛾Helicoverpa assulta( Hass)雄虫触角中扩增得到了信息素结合蛋白3( HassPBP3)。
- The cDNA Cloning of Pokeweed Antiviral Protein Gene and Research on Gene-transforming Tobacco[J].Journal of Henan Agricultural University,2006,40(2):130-132,141. PAP基因cDNA克隆及烟草转基因研究[J].;河南农业大学学报;2006;40(2):130-132;141
- The full-length cDNA clone, NDV3GM122, and the three helperplasmids pCI-NP, pCI-P and pCI-L of Newcastle disease virus strain ZJI isolated from an outbreak in the goose were cotransfected into BSR-T7/5 cell expressing T7 RNA polymerase. 利用反向遗传操作技术,将ZJI株鹅源新城疫病毒全基因组cNDA克隆(NDV3GM122)和含该毒株NP、P及L基因的3个表达载体(pCI-NP、pCI-P与pCI-L)共转染BSR-T7/5细胞;
- The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR. 在此基础上，将含有多克隆位点和编码NIa蛋白酶识别位点的Adaptor通过PCR介导插入到芜菁花叶病毒CDN1株系全长cDNA的NIb与CP区之间的NIa蛋白酶识别位点之后，将芜菁花叶病毒CDN1株系全长cDNA侵染性克隆pVIR95T改造成一个可利用的基因插入型表达载体pVIR95TM。
- full-length genomic cDNA clone of Gx strain Gx株全长cDNA
- infectious full-length cDNA clone 感染性全长cDNA克隆
- Forty-four cDNA clones, representing 44 defense-responsive genes, were fine mapped to 56 loci distributed on 9 of the 12 rice chromosomes. 44个cDNA克隆代表44个抗病相关基因被定位于水稻12条染色体中的9条染色体上的56个位点。
- The availability of cDNA clones for EcR and USP from several insect species has opened up possibilities for the development of high throughput EcR/USP based screening assays. 多种昆虫蜕皮激素受体和超气门蛋白基因的成功克隆为建立基于昆虫蜕皮激素受体的高通量筛选技术奠定了基础，并且可以通过多种途径实现。
- Betaine aldehyde dehydrogenase (BADH) cDNA cloned from Atriplex hortensis L. was transferred into tetraploid clone of black locust (Robinia pseudoacacia L.) by agrobacterium (Agrobacterium tumefaciens Conn) mediated transformation. 利用农杆菌(Agrobacterium tumefaciens Conn)介导法;将山菠菜甜菜碱醛脱氢酶(BADH)基因转入四倍体刺槐(Robinia pseudoacacia L.;)
- Rescue of VSV-Ind strain from cDNA clone by reverse genetic system 水泡性口炎病毒印第安纳株反基因操作系统的建立
- CDNA CLONING HUMAN TPA GENE cDNA FROM HUMAN EARLY STAGE EM BRYO 从人早期胚胎中克隆组织型纤溶酶原激活因子基因
- Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated. cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.;5-2
- Methods Full length HMGB1 cDNA cloned by PCR from mammary library was inserted into pET28a(+) vectors to construct pET28a(+)-HMGB1 plasmid. After confirmation, the positive pET28a(+)-HMGB1 recombinant plasmid was transformed into E. 方法应用PCR从乳腺文库中扩增得到HMGB1基因片段,并将其克隆到pET28a(+)载体上,对获得的pET28a(+)-HMGB1重组质粒扩增后进行PCR、酶切和测序鉴定。
- Two EST(One come from hemin induced HEL cells while the other come from PMA induced HEL cells) were chosen as the probes for cDNA library screening and several full length cDNA clones were isolated from human bone marrow cDNA library. 选择两个新EST筛选人骨髓细胞cDNA文库，获得两个新的锌指蛋白编码基因的全长cDNA，分别命名为HZF1(其EST来源于被hemin诱导的HEL细胞)和HZF2(其EST来源于被PMA诱导的HEL细胞)。
- CDNA Cloning of Myostatin Gene in Rabbit and Primary Analysis 兔肌肉生长抑制素基因的cDNA克隆及初步分析
