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- The fragments were cloned with pGEM-T easy kit and sequenced by the chain termination reaction. 结果在第4轮杂交及扩增反应后,获得4条差异性条带。
- chain termination reaction 链终止反应
- Incorporation of a dideoxy analog prevents further elongation and so produces a chain termination extension product. 双脱氧类似物的插入将阻止进一步延伸,而产生链终止延伸产物。
- The effect of residence time on the mechanism of chain termination of An freeradical polymerization was discussed. 探讨停留时间对丙烯腈聚合反应自由基链终止方式的影响。
- The oxidation state of Ti which is catalytically active to styrene syndiotactic polymerization is trivalent state.No termination reaction occurs within the active growing cha... 配位聚合时,苯乙烯分子是按二级插入方式进入分子链的,间规序列的形成是活性链末端苯基与前来配位的苯乙烯苯基相互排斥作用的结果。
- Hepsera slows the progression of chronic hepatitis B by interfering with viral replication and causing DNA chain termination after its incorporation into viral DNA. 通过干扰病毒的复制来减缓慢性乙肝的病变进程,和通过与病毒DNA整合来终止DNA分子链。
- The cDNA encoding the extracellular domain of human costimulatory molecule B7 1/CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method. 用RT PCR法从人外周血单核细胞克隆了编码共刺激分子B7 1 /CD80胞外区的cDNA ;并用Sanger链终止法进行测序 .
- Methods Total RNA extracted from rat brian cell was transcripted reversely into cDNA with random primers. The Glutamic acid decarboxylase 67 gene fragments were amplified using PCR method and sequenced using the dideoxy chain termination technique. 方法 采用RT -PCR方法 ;大鼠脑组织中的mRNA逆转录成cDNA ;再以cDNA为模板 ;扩增谷氨酸脱羧酶GAD6 7基因片段 ;克隆入T载体 ;并经序列测定 .
- The kinetoplast DNA of Trypanosoma evansi isolates, XJCA strain, AHB strain and YNB strain, were amplified by PCR, then the DNA sequences of the PCR products were analysed by the dideoxy chain termination method of Sanger. 对伊氏锥虫新疆骆驼株(XJCA)、安徽水牛株(AHB)和云南水牛株(YNB)的动基体DNA进行PCR扩增后,用Sanger双脱氧核糖核酸法测定扩增产物的序列。
- bimolecular termination reaction 双分子链终止反应
- chain termination method(Sanger method) 链终止(DNA测序)法
- METHODS:Cir- cumsporozoite protein(CSP) gene of P.vivax in Hubei was firstly sequenced by using rou- tine cloning and Sanger Chain Terminal method. 方法 :采用常规的克隆和 Sanger链终止法 ,首次对湖北省间日疟原虫的环子孢子蛋白 ( CSP)基因测序。
- Methods: The objects consisted of 57 patients with malignant pleural effusion and 57 patients with tuberculous effusion. CEA mRNA,TA and TB DNA were detected by PCR and ADA was detected by terminal reaction of enzymology. 方法:对114例恶性胸水(恶性组)和结核性胸水(结核组)患者以采集胸水为标本,应用PCR技术检测CEA mRNA、TA和TB DNA、酶动力学反应终点法检测ADA。
- The major use for Tert-dodecyl mercaptan is in the production of rubbers,fibers and resins,where it is used as a chain terminator. 叔十二硫醇主要用作橡胶、树脂及纤维生产中控制相对分子质量的链调节剂。
- Could I ask for your reaction to this policy? 我能问一下您对这个政策的反应吗?
- A reactor is a container for chemical reaction. 反应器是进行化学反应的容器。
- The chain has fallen foul of plants in the water. 链条已和水中的植物缠绕在了一起。
- She has a gold chain about her neck. 她在脖子的周围挂着一根金项链。
- I'm displeased at your reaction. 我对你的反应很不满意。
- The point at which this termination occurs. 停烧时刻这种时期发生的时刻