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- Methods The full length of CD95 cDNA was inserted into the multiple cloning site of the expression vector pBK CMB by molecular cloning technique. 方法 采用分子克隆技术将CD95基因插入真核表达载体pBK CMV的多克隆克隆位点之间 ,以脂质体介导法将目的基因导入受体细胞SGC790 1,用G418筛选克隆细胞 ;
- In the end, an expression T vector pBV-HX, which is the first practical use of the HphI-XcmI linker as a universal cloning site, was constructed by using the linker insert into vector pBV220. 将HphI-XcmI接头接入pBV220中构成一表达T载体是该接头的第一个实际应用。
- METHODS: The HAb18GEF gene fragment was amplified by PCR and digested with Sac I/Not I,and then cloned into the multiple clone site downstream of EpoR/LR-F3 gene of eukaryotic expression vector pCEL2f. 方法:用PCR法扩增肝癌相关抗原HAb18G胞外段基因,SacI/NotI双酶切后插入真核表达载体pCEL2f中,构建EpoR/LR-F3/HAb18GEF嵌合受体基因的重组表达载体pCEL2f/HAb18GEF,并经酶切和测序验证。
- Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively. 方法 :从 13d鸡胚骨骼肌mRNAs经RT PCR方法扩增RTEF 1的编码序列 (cDNA) ,将其克隆到 pGEM和 pUC载体并进行核苷酸序列分析。
- The monument was moved bodily to a new site. 纪念碑整个迁到一个新场址。
- The products of PCR werenamed Sa and Sb, of 2.1Kb and 2.3Kb respectively. Sa was inserted in EcoR I andKpn I sites after Sb was cloned in KpnI and PstI multiple cloning sites of the samepUC18 plasmid. 1Kb和2.;3Kb两个片段,经酶切鉴定后,先后插入到pUC18质粒载体上的EcoRI和PstI多克隆位点上,构建了重组质粒pUC-S。
- Is it safe to site the power station here? 在这建造发电厂安全吗?
- The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to construct an attenuated superantigen expression vector pLXSN SEA (D227A) linker CD80tm modulated by cis acting element of AFP gene. 将上述片断插入逆转录病毒载体 pLXSN的多克隆位点 ,构建AFP基因顺式作用元件调控的肝癌特异性减毒超抗原表达载体 (pLXSNSEA(D2 2 7A) linker CD80tm)。
- The group goes on a lecture tour of site in greece. 该旅游团前往希腊著名景点进行修学旅游。
- Excavation of the site will begin tomorrow. 明天开始挖掘工作。
- A dump truck was parked on the construction site. 建筑工地上停著一辆倾卸车。
- This mountain was the site for a quarry. 这座山曾经有一个采石场。
- They quickly leveled off the site. 他们迅速地把工地整平了。
- Our school has a good site in town. 我们学校位于镇上一个好地点。
- The local council intends to build on this site. 地方议会打算在这块地上盖房子。
- The site of the battle of Waterloo is in Belgium. 滑铁卢战役的遗址在比利时。
- All visitors to the site must wear safety helmet. 到工地的所有参观者必须带上安全帽。
- Wait for foster mother to give birth to the clone. 等待“养母”产下克隆幼仔。
- Will 2003 be the year of the first human clone? 人类第一个克隆人会在2003年诞生吗?
- A site used for or reclaimed by such disposal. 垃圾填筑地在上面使用这种方法的或用这种方法平整出来的地