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- Comet assay detects DNA single-strand breaks of single cell. 彗星试验研究和测定的是单个细胞DNA链断裂。
- By using MTT assay and Comet assay,the effect of neural stem cells on damaged spinal cord neurons was studied. 结果MTT比色试验表明实验组神经元活力明显高于对照组,彗星试验表明实验组拖尾率和彗尾长度均低于对照组。
- The anti-mutagenicity of reduced coenzyme NADH was studied by Ames assay and single cell gel electrophoresis assay (SCGE, comet assay). 采用Ames实验及单细胞凝胶电泳(SCGE,彗星实验),对还原型辅酶NADH进行抗突变研究。
- K562 cells were irradiated by low dose UVC, the DNA damage was detected by comet assay after treated 2h but not immediately. 低剂量UVC照射K562细胞经2h孵育后,彗星电泳可检测到即时检测不能发现的损伤,表明细胞的损伤具有延时效应。
- Among them,the wastewater from tannery had the strongest action of DNA damage.The results also revealed that the modified comet assay have br... 本研究显示出了改良彗星试验在综合评价各种废水遗传毒性方面具有广阔的应用前景。
- Apoptosis of immature thymocyte of mice induced by dexamethason and DNA strand broken cells induced by K 2Cr 2O 7 were tested by comet assay. 应用彗星试验对地塞米松诱导的小鼠胸腺凋亡细胞与重铬酸钾诱导的普通DNA链断裂损伤细胞进行了比较研究。
- Objective To compare the effect of Comet assay in peripheral blood lymphocytes and buccal mucosal cells on detecting genetic damage in workers exposed to acrylonitrile (ACN). 目的 比较外周血淋巴细胞和口腔脱落细胞彗星试验在丙烯腈作业人群遗传损伤评价中的效果。
- Methods KCl-SDS assay and comet assay were used to test the effect of DNA damages and the content of DPC induced by liquid formaldehyde in P815 cell line. 方法以P815细胞为材料,温箱孵育染毒1h后,采用单细胞凝胶电泳法和KCl-SDS沉淀法检测液态甲醛染毒后细胞中DNA的断裂效应及DNA-蛋白质交联物的含量。
- BHT could induce apoptosis to pulmonary macrophage in the perfusate of isolate perfused rat lung as examined by the technology of PI fluorescence stain and comet assay. 结论亚急性BHT大剂量染毒可造成大鼠肺、肝等脏器功能性和器质性损伤,BHT可诱发肺巨噬细胞凋亡的产生。
- Conclusion : The primary mice testicular cells had certain metabolic role and could be used to detect the effect s of DNA damage induced by indirect mutagen in comet assay. 结论:原代小鼠睾丸细胞具有一定的代谢活化作用,可以用于彗星试验 直接检测间接诱变剂所致的DNA 损伤研究。
- The DNA damage of spleen lymphocytes induced by three typical aldehyde pollutants was studied in vitro with microgel single cell electrophoresis assay (comet assay). 应用单细胞凝胶电泳技术(彗星实验)检测了3种典型醛类污染物对小鼠脾淋巴细胞DNA损伤情况。
- Method: Sperm DNA integrity of 40 semen samples from nonoligospermatism infertile men were detected by the using of comet assay before freezing and after thawing. 方法采用彗星试验检测40例非少精子症不育患者精液冷冻前及复苏后精子核DNA链的完整性。
- Through the lymphocytes of mouse spleen exposed single or jointed aldehydes,we examine the amount of DNA Strand Breakage (DSB) formation by comet assay. 应用单细胞凝胶电泳技术,通过对小鼠脾淋巴细胞染毒试验,测定了甲醛、乙醛、丙稀醛单独及联合作用下对细胞DNA分子链断裂损伤。
- Abstract Purpose : To explore the time-course effect s in detecting DNA crosslinks with comet assay and to prove the feasibility of comet assay for detecting DNA crosslinks. 摘要 目的:探讨用彗星试验检测DNA 交联剂作用的时间 效应关系及验证其应用的可行性。
- Objective To investigate correlation between nuclear DNA degradation of the skeletal muscle cells of rat detected by Comet assay and postmortem interval (PMI). 目的探讨彗星电泳检测大鼠死后骨骼肌细胞核DNA降解碎片与死后时间的关系。
- Abstract: Purpose and Methods: In this study, the micronucleus test and comet assay of Vice faber were used to screen pollution situation of the surface water of Yangzhong city. 文摘:目的与方法:本文应用蚕豆根尖微核技术和彗星试验对扬中市不同水体的遗传毒物污染状况进行了初步调查。
- The single cell gel electrophoresis(SCGE),also called comet assay,is a sensitive and rapid method for DNA damage detection in individual mammalian cell. 单细胞凝胶电泳是一种在单细胞水平检测哺乳类有核细胞DNA断裂的新技术。
- Single cell gel electrophoresis(SCGE) or comet assay(CA) is a rapid,simple,sensitive,reliable and inexpensive method for detecting DNA damage in eukaryotic individual cells. 单细胞凝胶电泳(SCGE)是一种快速、简便、灵敏、可靠的在单细胞水平上检测真核细胞DNA损伤与修复的技术。
- When assessing the early genetic damage of ACN exposed population during health surveillance,Comet assay in peripheral lymphocytes was more sensitive than that in buccal mucosal cells. 对丙烯腈接触人群进行遗传损伤监护时 ,外周血淋巴细胞彗星试验比口腔脱落细胞彗星试验更灵敏。