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Since constitutive expression system is more likely leading to the loss of plasmid compared with inducible system,enhancing the plasmid stability of a constitutive system is more difficult. 与诱导型表达系统相比,在组成型表达系统中,由于外源蛋白的持续表达,导致细胞代谢负担加重,质粒稳定性的控制更加困难。
constitutive expression system 组成型表达系统
Keywords penicillin G acylase;constitutive expression system;plasmid stability;glucose; 青霉素G酰化酶;组成型表达系统;质粒稳定性;葡萄糖;
Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
Objective. To determine the constitutive expression and production of NGF and the effect of cytokines on the expression of NGF by human IVD cells. 目的:判断NGF的结构性表达以及产生;并人类椎间盘细胞的细胞因子对于NGF表达的作用.
The advantage of constitutive expression vectors is that we need not feed mice with nisin, which makes the experiment on orally taken viccine in LAB more easily. pNZ8037能在乳酸乳球菌、乳酸杆菌、枯草芽孢杆菌等多种革兰氏阳性菌和大肠杆菌中稳定复制、存在,是一个广泛宿主范围的质粒。
Single-chain Fv has mature prokaryocyte expression system with high yield, but its stability is poor. 单链抗体有较成熟的原核表达系统，产量较高，但稳定性较差。
To improve this, the thesis researched the non-lytic baculovirus expression system by two different ways.First, Dr. 为了改善这个缺点，本论文由两个方向研究非溶裂杆状病毒表现系统。
Methlotrophic yeast and fission yeast S.pombe have received attention as a good foreign genes expression system. 甲醇营养型酵母和裂殖酵母作为外源基因表达的有效系统,正引起人们广泛研究。
To improve this, the thesis researched the non-lytic baculovirus expression system by two different ways. 为了改善这个缺点，本论文由两个方向研究非溶裂杆状病毒表现系统。
Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
Low constitutive expression of TnIL-21 was observed in limited tissues of healthy fish including gut, gill and gonad. Stimulation with LPS significantly induced the expression of TnIL-21 in kidney and spleen. TnIL-21仅在正常鱼体组织的鳃、肠和性腺中有少量表达，LPS刺激能诱导TnIL-21在头肾和脾脏中表达。
We have launched a new product line, NICE? Expression System for Lactococcus lactis. 我们现在开发了一条新的产品线----乳酸乳球菌表达系统
The MP fragments were then fused with constitutive expression promoter CaMV 355 and pathogen - inducible promoters CHS, Pill and BG, respectively, which led to the consmiction of plant expression vectors p355-30K, pPiII-30K, pPiII-30K and pBG-30K. MP片段分别与组成型启动子CaMV35S和病原特异诱导型启动子CHS、PiII和BG体外重组，构建成植物表达载体P35S－30K，pCHS－30K，pPiII－30K和pBG－30K。通过农杆菌LBA4404介导，分别转化含Tin－22抗性基因的番茄品种Geneva80。
Pichia pastoris expression system is a recently developed system to efficiently express foreign protein. 摘要毕赤酵母表达系统是近年来发展起来的一种高效表达外源基因的表达系统。
The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
The invention provides a CD86 humanized monoclonal antibody, an associated gene order and an expression system thereof. 本发明提供一种CD86人源化单克隆抗体，其相关基因序列和表达系统。
Mechanism of Cardiac Sarcolemmal Adenosine Triphosphate-sensitive Potassium Channel Activation by Isoflurane in a Heterologous Expression System. 异氟醚激活异源性表达系统中的心脏胞浆。
Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。