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- Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. 分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。
- SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes. SOX基因兼并引物延伸发现了更多的SOX基因位座,并进一步证实该家族基因在基因组中是散在存在的。
- Five degenerated primers were designed according to the P-loop, Kinase-2a, Kinase-r3a, EGF domain of NBS which universally exist in cloned R genes. PCR amplifications were performed with the designed RGA primers and cDNA as templates. 根据已克隆R基因普遍具有的NBS结构域的P-loop、Kinase-2a、Kinase-3a、EGF区设计5条RGA简并引物,组成5对引物组合。
- Keywords Bacillus fastidious uricase;degenerated primer PCR;kinetic uricase method for serum uric acid assay;xanthine interference;gene cloning; 关键词苛求芽孢杆菌尿酸酶;血清尿酸测定专利尿酸酶法;黄嘌呤干扰;简并引物PCR;基因克隆;
- Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a general primer containing oligo-dT). N-端 9个氨基酸的序列,并据此设计5,端简井引物,用此引物和 3’-端通用引物,通过RTFCR技术克隆了 Luffin S。 的部分cDNA序列。
- He ate up Nietzsche and degenerated into a fascist. 他接受了尼采的思想,堕落成法西斯分子。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- degenerated primer 简并引物
- Methods The erm genes of MRCNS were detected by degenerate primers amplification. 方法 应用兼并引物扩增MRCNS菌的erm基因。
- The SOX genes of Panthera tigris altaica Temminck were detected by a pair of degenerate primers. 应用特异于HMG bOX区域的兼并引物 ,扩增了东北虎的SOX基因。
- He has degenerated into an alcoholic. 他堕落成酗酒者。
- In This study, a set of degenerate primers were designed according to the conserved amino acid sequences of CYP6A1, CYP6DI in Musca domestia and CYP6E1 in Culex quinquefasciatus. 根据家蝇CYP6A1、CYP6D1以及致倦库蚊CYP6E1的保守区氨基酸序列设计一组简并引物,采用RT-PCR的方法,从白纹伊蚊四龄期活体幼虫总RNA中扩增出与设计相符的基因片段。
- A coat of primer should be made over the bare surface before the main painting. 在喷涂主漆前,应在物品的表面刷一层底漆。
- I borrowed a primer of science from the library. 我从图书馆借了一本科学的入门书。
- All animals degenerated in America. 一切动物到了美洲就要退化。
- The argument degenerated into a row. 争论变成吵架。
- Her commitment to a great cause degenerated from a crusade into an obsession. 她致力于一伟大事业,但其崇高的奋斗精神已变质成为偏执的狂热。
- South family store has a 'Degen'. 家乐南楼店有个‘德根’。
- Spray primer to see the condition of surface. 喷水补土检查表面状况。
- I am the sun: So like carbon black Degen. 我很阳光:所以黑得跟炭似的。