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donor cells gene transfection 供体细胞转染
The phage has replicated and the donor cells have lysed. 噬菌体已复制和给体细胞已发生裂解。
Objective To study the effect of ULBP3 gene transfection on the sensitivity of Saos-2 cell line to human NK killing. 目的观察ULBP3基因转染对人外周血NK细胞对Saos -2细胞杀伤活性的影响。
ULBP3 gene transfection make Saos-2 cell line more sensitive to human NK killing. ULBP3基因转染能提高人外周血NK细胞对人成骨肉瘤Saos-2细胞的杀伤活性。
After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
Using such an early donor cell might avoid the risk of accumulated DNA damage. 使用这样的一个早期供体细胞可能避免积聚DNA损伤的风险。
Purpose To study the effects of aldose reductase (AR) gene transfection and AR inhibitors (ARI) on the proliferation of rat mesangial cells (MsC) in vitro. 目的观察醛糖还原酶(AR)基因转染及AR抑制剂(ARI)对体外培养大鼠肾系膜细胞(MsC)增殖的影响。
And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
In general idea, cumulus cell is the best nuclear donor cell for reprogramming. 一般来说，颗粒细胞作为核供体最易被核重编程。
Objective To explore the feasibility and the ettects of human BMP-2(hBMP-2)gene transfection on bone mesenchymal stem cells(BMSCs)from osteoporotic rats in vitro. 目的探讨人骨形成蛋白2(human bone morphogenetic protein-2,hBMP-2)转染骨质疏松症大鼠骨髓间充质干细胞(bone mesenchymalslem cells,BMSCs)的可行性及其对细胞生物学行为的影响。
Meantime, the qualitative observation demonstrate that the proliferation of the smooth muscle cells with the eNOS gene transfection was inhibited through the NO. 同时，实验通过定性观察验证了转染eNOS基因可以通过产生NO，抑制血管平滑肌细胞的增殖。
Conclusion: PTEN gene transfection combined with L-OHP has obvious inhibitory effect on cholangiocarcinoma cell line in vitro. 结论：PTEN基因转染生物治疗联合奥沙利铂化疗对人胆管癌细胞生长具有显著的抑制作用。
Donor cells play a very important role in nuclear transplantation of Somatic Cell. 供体细胞在体细胞核移植中发挥着非常重要的作用。
The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
Ultrasound can increase cell permeability by cavitation, so that it can improve the efficiency of gene transfection and transgene expression in cells in vitro and in vivo. 超声通过其空化效应可增加细胞膜的通透性,进而能促使外源基因进入细胞,增强了基因在体外细胞和体内细胞的转染和表达水平。
We could conclude that culture and passage of donor cells might be benefit to nucleus reprogramming. 体外培养传代有利于体细胞核移植后重新编程。
This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. 摘要本研究旨在提高基因转染人红白血病细胞株K562的转染效率，同时观察绿色荧光蛋白(GFP)作为报告系统用于转染条件优化的可行性。
On the contrast, somatic donor cells showed high percentage of methylation (66% and 63% in FFB and FOV cells, respectively). 然而;在体细胞中;Xist基因的甲基化程度较高;FFB为66%25;FOV为63%25.
Using a new technique developed in Padua University, the trachea was decellularised over a six-week period so that no donor cells remained. 利用帕多瓦大学研发的一种新技术，对该气管进行了六个多星期去细胞处理，以至于不会残留供者细胞。