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- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法：用地高辛素标记基因探针，斑点杂交法检测未知和已知病原的肝炎患者75例，正常对照17例。
- Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑点杂交技术和免疫组化染色测定40例正常前列腺(NP)、38例前列腺增生症(BPH)组织中b-FGF的表达情况。
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
- After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method. 传一代细胞以10mg/ml复方861作用48小时后 ,以ELISA法、斑点杂交法检测胶原蛋白分泌及相应mRNA水平的变化。
- Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分别对不同来源的猪细小病毒DNA及PUP重组质粒于硝酸纤维素膜上打点杂交，免疫呈色后均为阳性反应，而对照的猪瘟病毒、乙型脑炎病毒、伪狂犬病毒、PK－15细胞的核酸均为阴性反应。
- Dot blot hybridization with the two probes showed the positive result for PPV DNA,but negative for the nucleic acid samples obtained from Hog cholera virus,Pseudorabies virus,Japanese B Encephalitis virus and PK 15 cells. 对猪细小病毒DNA进行斑点杂交，两种探针均为阳性，而对照的猪瘟病毒、猪伪狂犬病毒、乙型脑炎病毒及PK－15细胞的核酸均为阴性。
- Keywords mycobacteriua;atypical;polymerase chain reaction;dot blot hybridized;microarray; 分支杆菌;非典型性;聚合酶链反应;斑点杂交;微阵列;
- dot blot hybridized 斑点杂交
- The cDNA probe hybridized with BVDV RNA, HCV RNA and yeast tRNA by the dot blot method The BVDV RNA and HCV RNA were positive and yeast tRNA was negative. 琼脂糖凝胶电泳结果说明它与BVDV RNA相似。 该cDNA的探针用斑点杂交方法与BVDV RNA,HCV RNA和酵母tRNA杂交,BVDV RNA和HCVRNA显阳性反应,酵母tRNA显阴性反应,结果说明合成的cDNA为BVDV RNA的cDNA。
- SCREENING MUTATIONS IN PHENYLKETONURIA BY MEANS OF NONRADIOACTIVE REVERSE DOT BLOT HYBRIDIZATION 非同位素逆相点杂交方法检测苯丙酮尿症突变基因
- Rapid Detection of Mycobacterium Tuberculosis Streptomycin-resistant Genes by PCR-reverse Dot Blot Hybridization 应用PCR-反向斑点杂交技术快速检测结核分枝杆菌耐链霉素基因型
- Rapid Detection of Mycobacterium tuberculosis Ethambutol-resistant Genes by Membrane-reverse Dot Blot Hybridization 应用膜反向斑点杂交技术快速检测结核分支杆菌耐乙胺丁醇基因型的研究
- Rapid detection of mycobacterium tuberculosis isoniazid-resistant genes by membrane reverse dot blot hybridization 应用膜反向斑点杂交技术检测耐异烟肼结核分支杆菌基因型
- USE OF ELISA AND cDNA DOT BLOT HYBRIDIZATION IN DETECTION OF BARLEY YELLOW DWARF VIRUS AND STRAIN INTERACTIONS 用cDNA点杂交及ELISA测定大麦黄矮病毒及分子间互作
