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- Effect of interferon on HBV determined by dot hybridization and PCR. HBeAg、HBV DNA斑点杂交以及定量PCR检测在评价干扰素抗HBV疗效的价值探讨
- EGFR gone transcription in rat embryo and adult tissues were analysed by RNA Northern and Dot hybridization using 2.4 Kb human EGFR eDNA pE 7 as probe. 本文报告用2.;4Kb EGFR cDNA PE7为探针;分析大鼠胚胎发育过程中全胚组织、成年大鼠七种组织和再生肝组织中EGFR基因转录产物的水平。
- Objective To evaluate specimen processing with sodium dodecyl(lauryl) sulfate(SDS) on the detection of Mycobacterium tuberculosis by PCR-reversed dot hybridization. 目的探讨应用十二烷基硫酸钠(SDS)处理标本在结核分支杆菌聚合酶链反应(PCR)中的价值。
- Using biotinylated human Y-specificDNA probe PHY2.1 and Cos 84, we carriedout Southern and dot hybridization withhuman DNA from artificially aborted cho-rionic villi. and with DNA of peripherallymphocytes from normal male and female. 作者以流产绒毛和外周血淋巴细胞DNA为材料;通过Southern印迹杂交和点杂交;考察了用生物素标记Y特异DNA探针PHY2.;1和Cos84进行产前性别诊断的可行性。
- Though RNA dot hybridization is negative, OP J18-1400 has a conservative domain of seven base pairs DNA sequence: 5-TTCCCTC-3, which is a homologous recombination hotspot domain in atp6 gene. 虽然OPJ18-1400对应的RNA斑点杂交呈阴性,但其DNA序列中存在一个七碱 基5-TTCCCTC-3的保守系列,它是atp6基因中同源重组热点区,其促使线粒体基因重组形成嵌
- However, by RNA dot hybridization the amount of mRNA produced in the transcription of parg-leuS was about 5 times that of the wild type le uS, and was similar to that of pKK-leuS, suggesting that the promoter of argS is very strong. 但是 ,RNA斑点杂交却发现从 parg leuS转录出来的leuSmRNA是野生型leuS转录的 5倍 ,几乎与从 pKK leuS中转录出来的量差不多 ,说明argS的启动子很强 ,接近于trp lac。
- The two recombinant plasmids were transfected into the HepG2 cells , then the inhibition effect on HBV replication by the genome of the HBV defective virus was studyed by ELISA dot hybridization and semiquantitative PCR. 构建含乙肝缺陷病毒基因组的重组逆转录病毒载体和含乙肝病毒基因组的重组逆转录病毒载体,共转染HepG2细胞,ELISA、PCR方法和斑点杂交初步研究乙肝缺陷病毒基因组对HBV复制的影响。
- Dot hybridization technique in diagnosis of condyloma acuminata HPV核酸探针斑点杂交技术在诊断尖锐湿疣中的应用
- Keywords rotavirus vaccine;gene transmit;dot hybridization; 轮状病毒疫苗;基因转化;斑点杂交;
- The train arrived on the dot of three o'clock. 那辆火车三点钟准时到达。
- He said he used this system in the year dot. 他说他很久以前用过这个系统。
- He went away, limping dot and go one. 他一拐一拐地跛着走开了。
- Detection of -Sugarcane mosaic virus- by dot hybridization and RT PCR technique 利用斑点杂交法和RT-PCR技术检测甘蔗花叶病毒
- He's very punctual always arrives on the dot. 他很守时--总是按时到。
- The hybrid offspring of a zebra and a horse. 杂交斑马斑马与马杂交的后代
- She was told to arrive at 8 o'clock on the dot. 她被告知八点钟准时到。
- A mule is a hybrid of a donkey and a horse. 骡子是公驴和母马交配而生的杂种。
- That is a hybrid perpetual rose. 那是一株杂种的四季开花的蔷薇。
- A mule is a hybrid of a male donkey and a female horse. 骡子是公驴和母马交配而生的杂种动物。
- He watched the train away until it was only a dot in the distance. 他目送着列车驶去,直到它成了远方的一个小黑点。