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- Functional genomics: Gene identification via T-DNA mediated gene trap tagging in plants 工能基因组学:T-DNA介导的基因诱捕和植物基因鉴定
- Gene trap elements are alsoused in the construction of retrovirus vectors. 基因诱捕技术也被运用于反转录病毒载体的构建。
- Gene trap elements are also used in the construction of retrovirus vectors. 基因诱捕技术也被运用了反转录病毒载体的构建。
- ATF-lys10 mediated gene delivery is uPAR targeting, and the preparation of gene delivery complex is relatively simple. ATF-lys10介导的基因转移具有uPAR靶向性,而且基因转移复合物制备相对比较简单。
- Stable Integration of Genomic Gene of Interest in Mammalian Cells Following Adeno-associated Virus Vector Mediated Gene Transfer. 腺相关病毒介导基因组来源的基因转移与整合。
- Objective To study the mechanism of bystander effect in herpes simplex virus thymidine kinase(HSV TK)/Ganciclovir(GCV) mediated gene therapy. 目的 探讨用单纯疱疹病毒胸腺嘧啶核苷激酶(HSV?TK)/丙氧鸟苷(GCV) 系统进行基因治疗时发生的旁观者效应机制。
- The strategies of large-scale mutagenesis and gene screening include chemical mutagenesis, insertional mutagenesis and gene trap. 摘要用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- The strategis of large-scale mutagenesis and gene screening include chemical mutagenesis,insertional mutagenesis and gene trap. 摘要 用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。
- Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy. 结果表明:在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中,IRES与内部SV40启动子相比,更能保证双基因的共同表达。
- Retrovirus mediated gene transfection was carried out. First, the package cell line PT67 was transfected by lipofectAMINE TM2000, so as to obtain recombinant retrovirus supernatant, which was directly used in the transfection of MSCs. 反转录病毒介导的基因转染,首先利用LipofectAMINETM 2000转染包装细胞系PT67,获得重组反转录病毒上清,然后用病毒上清直接感染骨髓间充质干细胞。
- METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method. 方法 :将含有肝癌相关抗原HAb1 8G全长cDNA的真核表达载体 pcDNA3.;0 /HAb1 8G利用脂质体介导法转染小鼠成纤维细胞NIH/ 3T3;G4 1 8筛选建立稳定表达阳性克隆细胞株t3T3; 利用流式细胞仪、间接免疫荧光染色方法鉴定目的蛋白转染情况 ;
- Lu Deyang,Fan Yunliu,Yu Meimin,Tang Shunxue,Hou Ning,WANG Qing.Regeneration of HNP transgenic alfalfa plants by Agrobacterium mediated gene transrer[J].Acta Genetica Sinic,2000,27(4):331-337. [3]吕德扬;范云六;俞梅敏;唐顺学;侯宁;汪清.;苜蓿高含硫氨基酸蛋白转基因植株再生[J]
- Gene Trapping Techniques and Current Progress 基因捕获技术及其最新进展
- Gene Trap Techniques and Gene Trap Resources 基因诱捕技术及基因诱捕数据库
- chromosome mediated gene transfer CMGT
- In a printer, the removable type element. 印刷机上可拆卸的打印部件。
- chromosome mediated gene transfer(CMGT) 染色体介导的基因转移
- He's in his element when playing badminton. 他打起羽毛球来真是得心应手。
- Objective:To investigate the efficiency of cationic liposome in mediating gene transfer. 目的:研究阳离子脂质体介导体内外基因转移的效率。