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Protoplast The protoplasm and plasma membrane of a cell after removal of the cell wall, where present. This can be achieved by physical means or by enzymic digestion. 原生质体：指去掉细胞壁以后的细胞原生质以及质膜。可用物理方法或酶消化得到。
The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Cationic colloidal gold (CCG) probe and various enzymes digestion on CCG labelling were taken for the study. 采用阳离子胶体金(CCG)探针和系列酶消化包埋后标记法观测。
Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. 蛋白胨是一种酶消化的蛋白质用来培育非挑剔的有机体，生产低级别的增长。
Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法，但是也都有各自的缺点。
Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
Method:The olfactory receptor neurons could be isolated from olfactory mucous of Kunming mouse using enzymatic digestion and mechanical isolation. 方法利用酶消化结合机械吹打方法,急性分离昆明小鼠嗅粘膜的嗅感觉神经细胞。
The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶／DNA酶消化法进行消化，经光镜和电镜观察。
The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
The recombination vector that was named as pCAM-P was obtained based on the tests of PCR and enzyme digestion of the vector. 2. PCR检测、质粒酶切分析结果表明获得了Pto与pCAMBIA1300的重组子，命名为pCAM-P。
SCs were isolated by warm enzyme digestion, tissue culture, cold enzyme digestion of substep and cold enzyme digestion of combine. 雪旺细胞的分离采用了组织块法、热酶消化法、冷酶分步消化法和冷酶联合消化法。
The study on enzymatic digestion of collagen in recent years were described. The application prospect of the collagen polypeptide was analysed. 阐述了近年来国内外关于酶法水解胶原蛋白所做的研究工作,并对胶原多肽的应用前景进行了分析。
Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
BACKGROUND: At present, the preparation methods of acellular vascular matrix include enzyme digestion and decontaminant. 背景：目前脱细胞血管基质的制备方法有酶消化法和去污剂法。
Alkali treatment is the antithesis of enzyme digestion, for it is harsh rather than gentle, nonselective rather than specific, and cheap rather than expensive. 碱处理法和酶消化法相反，反应激烈，不具选择性，而且较便宜。
Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。