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eukaryotie expressing vector 真核表达载体
eukaryotie expression vector 真核表达载体
Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
Finally a reshaping scFv 2F3 library were constructed by inserting whole scFv 2F3 gene into the secretive adhesion expressing vector pFUW80. 我们把整个单链抗体2F3基因插入到分泌型融合表达载体pFUW80中，建成了人源化单链抗体2F3基因库。
Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题：图1.;穿梭表达载体pRL_hEGF的构建。
The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
Objective To construct the antisense nucleic acid eukaryotic expressing vector of the cDNA sequence encoding the transmembrane domain of receptor tyrosine kinase RON (RON-RTK) gene (RONm). 目的构建RON基因跨膜区段(RONm)的反义核酸真核表达载体。
The eukaryotic expressing vector encoding human cbl is constructed and it expresses flag-cbl fused protein correctly in COS-7 cells. 成功构建了N 末端带flag标签的cbl真核表达载体 ;并使其在真核细胞COS 7中表达 .
The recombinant plasmid pMD-ORF2 was digested with EcoR V and Xhol I and then the fragment was sub-cloned into the prokaryotic expressing vector pET-32a(+), getting the recombinant plasmid pET-ORF2. 将重组质粒pMD-ORF2的EcoR V和Xhol I双酶切产物亚克隆到原核表达载体pET-32a(+)，构建ORF2基因重组表达质粒pET-ORF2。
The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3). 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;
Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
Objective: To construct a procaryotic expression vector of vascularendothelial growth factor 165 (VEGF165) and to express VEGF165 inE. coli. 目的:构建人血管内皮生长因子 165(Vascular endothelial growthfactor 165,VEGF165)原核表达载体并在大肠杆菌中诱导表达。
Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
Eukaryotic expression vector pCDNA3-AADC can be efficiently expressed in primary muscle cells. 含人类神经元AADC基因的真核表达重组体可在原代培养的骨骼肌细胞中有效的表达。