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- MethodsThe optimized host cell,culture medium and induction time were deternined according to the growth and expression specificity of nrhTNF on test tube and flask shaker. Then fed batch culture was carried out on 5 L automatic fermentor. 方法通过探讨nrhTNF工程菌在试管和锥形摇瓶中的生长和表达规律 ,筛选并确定其最适宿主菌、最佳培养基及最佳诱导表达条件 ,然后在 5L自控发酵罐中进行分批补料培养。
- In fed batch culture,the activity of neutral protease was further increased to 17. 67 g/min L by controlling the feeding rates of glucose and other niedium components. 在控制补加葡萄糖和其它培养基成分的补料分批培养中,中性蛋白酶活性达17.;670g/mm·L。
- A new type of overflowing jetting photobioreactor was used for the indoor batch culture of Spirulina . 采用一种新型溢流喷射光生物反应器进行螺旋藻的室内分批培养试验。
- During the small vessel culture,batch culture and feeding culture in the fermenter,the characteristics of cell growth and metabolism of oxygen resistant Bifidobacterium bifidum were discussed. 分别讨论了耐氧两歧双歧杆菌在小瓶培养、发酵罐间歇培养以及流加培养的生长代谢特性,探讨了实现其高密度培养的途径。
- The effects of carbon-nitrogen mass ratio (C/N) on growth and hydrogen production were examined by batch culture with a strain of fermentative hydrogen producing bacterium Rennanqilyf3. 利用间歇培养研究了碳氮质量比对纯培养制氢工程中发酵产氢细菌产氢性能的影响。
- Study on the feed batch culture technology of Bt submerged fermentation 苏云金杆菌深层发酵补料分批培养工艺研究
- Study of Substrate Inhibition and Fed Batch Culture in The Fermentation of D-Ribose D-核糖发酵过程中底物抑制及补料的研究
- Keywords ALDC;fed batch culture;high density culture;engineering bacteria;extracellular enzyme; 乙酰乳酸脱羧酶;分批补料;高密度培养;基因工程菌;胞外酶;
- Keywords pyruvic acid;Torulopsis glabrata;glucose;initial glucose concentration;batch culture;fed batch culture; 丙酮酸;光滑球拟酵母;葡萄糖;初始葡萄糖质量浓度;分批培养;补料培养;
- Unwaged members pay a lower entrance fee. 无固定收入的人入场费从优。
- That boxer is a prodigious feed. 那个拳击家是个大肚汉。
- Keywords Torulopsis glabrata;pyruvic acid;nutritional conditions;ratio of C:N;fed batch culture; 丙酮酸;光滑球拟酵母;营养条件;碳氮比;流加培养;
- The ducks of the production team have been fed out. 生产队的鸭子已养肥到可以上市了。
- The cat looked very sleek and well fed. 这只猫毛很光滑,看来喂得很好。
- She charged only a nominal fee for her work. 她为所做的工作只收取象征性的费用。
- I'm fed up with all this argument. 我受够了这种争论。
- The second batch of sugar is better than the first. 第二批制成的糖比第一批好。
- I am fed up with his laziness and carelessness. 我受够了他的懒惰和粗心。
- The fee paid to a mint by a government. 铸币费政府支付给铸币厂的钱
- In batch or remote batch entry, a job or job step. 在批处理或远程批处理输入中的一个作业或作业步。