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- The results manifested that both BRL-CM and common medium were suitable for porcine EG cells culturing and passaging when having STO feeder cells. 结果表明,以STO细胞作饲养层,使用BRL条件培养基与常规培养基在猪EG细胞分离培养、传代后均可获得细胞集落,而且使用BRL条件培养基培养产生的集落数多,细胞生长速度快。
- Objective To investigate the preparation methods of feeder cells of mouse embryonic stem cell. 摘要目的:探讨小鼠胚胎干细胞饲养层细胞的制备。
- In order to optimize the culture conditions, human feeder cells and feeder-free, unconditioned culture systems have been developed. 为了优化培养条件,目前人们已发展了多种人类细胞饲养层和无饲养层、非条件培养基体系。
- Feeder cells with murine embryonic fibroblasts(MEF) were better than that with human embryonic fibroblasts(hEF), or STO or bovine embryonic fibroblasts (bEF). MEF、STO、bEF和hEF可提高人原始生殖细胞(PGCs)的贴壁率,能促进PGCs源的人类胚胎干细胞(ES)的分离效率; 以MEF作为饲养层效果较好。
- When goat primordial germ cells were cultured on different feeder cells,MEF,GEF and BEF all could sustain goat primary embryonic germ cells,and MEF was finer than others. 以小鼠胎儿成纤维细胞(MEF)、山羊胎儿成纤维细胞(GEF)、牛胎儿成纤维细胞(BEF)为饲养层,在不添加细胞因子情况下,都可以培养出原代山羊的胚胎生殖细胞。
- Limbal stem cells with feeder cells grew as clones. The mean CFE of limbal stem cells in the group with feeder cells was more than that in the group without feeder cells (t=2.982,P<0.01). 与饲细胞共同培养的兔角膜缘干细胞形成细胞克隆团;细胞的平均CFE比较:有饲细胞组角膜缘干细胞明显高于无饲细胞组(t=2.;982;P<0
- One EG cell line maintained undifferentiation for 6 passages. When bovine primordial germ cells were cultured on different feeder cells: MEF, STO and BEF, all can sustain bovine primary embryonic germ cells, and MEF was the best. 用不同的饲养层培养牛原始生殖细胞,实验结果表明MEF,STO和BEF饲养层都能支持牛原代PGCs的增殖,但MEF较BEF和STO效果更好以MEF、GEF、BEF为饲养层,在不添加细胞因子情况下,都可以培养出原代山羊的胚胎生殖细胞。
- When goat primordial germ cells were cultured on different feeder cells, MEF, GEF and BEF all can sustain goat primary embryonic germ cells, and MEF was finer than others, but there were no significant difference between MEF and GEF (P>0.05) . 传代结果表明,MEF的培养效果较好,但与GEF组二者差异不显著(P>0.;05),BEF组培养效果较差(P<0
- Rescue of Dying Adherent Cells by Feeder Cells 饲养细胞用于挽救濒死的珍稀贴壁细胞
- Keywords Co irradiation;PBMC;feeder cells; 关键词60钴照射;外周血单个核细胞;饲养细胞;
- Keywords Feeder cells;Adherent cells;Rescue; 饲养细胞;贴壁细胞;挽救;
- The little boy is a noisy feeder. 这个小男孩吃东西时发出响声。
- Keywords mouse embryonic stem cells;embryoid body;feeder cells; 小鼠胚胎干细胞;类胚体;饲养细胞;
- Keywords human embryonic cells;feeder cells;SNL cell line; 人胚胎干细胞;饲养层细胞;SNL细胞;
- Human tissue is made up of cells. 人体的组织是由细胞构成的。
- A tissue is a group of similar cells which have an identical general function. 组织是一组具有相同的一般机能的类似细胞。
- Animal bodies are made up of cells. 动物的身体是由细胞组成的
- The birds peeped exactly as we refilled the feeder. 当我们再加饲料的时候,小鸟就唧唧喳喳叫个不停。
- A small mass of tissue or aggregation of cells. 结组织的小结块或细胞的聚集
- Each LifeLine Fund is a feeder fund. 生命线基金是支线基金的一种。