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- 目的利用Bac-to-Bac杆状病毒表达系统,将人碱性成纤维细胞生长因子(human basic fibroblast growth factor,hbFGF)基因进行表达,并检测重组蛋白的特异性及生物学活性。Objective Bac-to-Bac baculovirus expression system was used to obtain biologically active recombinant human basic fibroblast growth factor(hbFGF) in insect cells.
- 目的 比较类风湿关节炎 (rheumatoidarthritis,RA)与骨关节炎 (osteoarthritis,OA)成纤维样滑膜细胞 (fibroblast likesynoviocytes,FLS)蛋白酪氨酸磷酸化状态的差异 ;Objective To evaluate the change of tyrosine phosphorylation status in rheumatoid arthritis fibroblast like synoviocytes (RA FLS),compared with osteoarthritis (OA) FLS;
- 目的应用细胞增生指标核仁组成区相关嗜银蛋白(argyrophilic nucleoar organ-izer regions granules,AgNORs),观察兔眼滤过道成纤维细胞(fibroblast,Fb)增生规律及三氧化二砷(arsenic trioxide,As2O3)的抗增生作用。Objective To investigate the proliferative regularity of fibroblasts(Fb) in the filtrate passageway of rabbit and observe the effect of arsenic trioxide(As2O3) on inhibiting the proliferation of Fb by taking argyrophilic nucleoar organizer regions granules (AgNORs) as a proliferative index. Methods Forty-eight rabbits were divided into experimental and control group randomly.
