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- Conclusions:E7 protein of HPV16 (HB strain) could be prepared by gene clone technique.HB-E7 protein displayed antigenicity of standard E7 protein. 结论：HPV16湖北株E7蛋白可通过基因克隆方法制备，并保留了标准E7蛋白的抗原性。
- In order to attain the complete protein sequence of APLA2-1, this study had also determined the eDNA sequence by gene clone method and therefore deduced the protein sequence. 为了得到APLA_2-1的蛋白质全序列，本研究还利用基因克隆的方法测定了APLA_2-1的cDNA序列，从而推导出蛋白质序列。
- The purpose of this paper is to review citrinin's physicochemical properties,toxicity,inhibiting bacteria activity,analytical methods,biosynthetic pathway,gene clone,etc. 本文介绍了红曲霉桔霉素的理化性质、毒性、抑菌性、检测方法、生物合成途径及基因克隆等内容。
- Methods:To clone and express E7 gene of HPV16 with gene clone technique. To pruify E7 protein and inoculate mice. The reactivity of special E7 antibody in sera was detected by ELIAS to evaluate antigenicity of E7 protein. 方法 :应用基因克隆技术克隆、表达HPV16E7基因 ,纯化E7蛋白并免疫动物 ,用ELISA检测动物血清中的特异性E7抗体 ,进而评估E7蛋白的抗原性。
- In the paper, studies on chitinase of microbes are briefly reviewed, and the classification, characteristics, purification, gene clone and expression, and its application in practice were recommended. 摘要概述了有关微生物几丁质酶的研究情况，介绍了几丁质酶的分类、性质、分离纯化、基因克隆与表达以及在实际中的应用。
- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- ACC Oxidase Gene Cloning and Sequence Analysis of Citrus aurantium L. 酸橙ACC氧化酶基因片段克隆及序列分析。
- This feature is favorable for gene cloning using transposon tagging strategy. 这个特性有利于转座因子作为分子标签克隆异源植物中的结构基因。
- Progress of CMS restoring gene clone of plant 植物细胞质雄性不育恢复基因的克隆研究进展
- Keywords LH;Gene clone;Plasmosome expression; 促黄体素;基因克隆;基因表达;
- Keywords QTLs;micronutrient;gene clone;wheat; 小麦;微营养素;QTL定位;基因克隆;
- Gene cloning provides a means of purifying and propagating specific DNA segments. 基因克隆化提供了一种纯化和扩增特定DNA片段的方法。
- ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis. 通过引物设计、PCR、基因克隆、测序,获得了10种鸢尾属植物和1个外类群种的ITS序列。
- This paper summarizes and illustrates the strategy of how to do EST analysis and gene cloning through bioinformatics. 本文通过实例综述了怎样运用生物信息资源进行EST筛选及其新基因克隆的策略。
- Objective： To screen for the gene clones with differential expression in normal liver and hepatoma. 目的 筛选肝癌与正常肝基因表达差异克隆。
- Abstract Development of biotechnique, especially molecular marker technique, lays the foundation for R gene cloning. 摘要 分子生物学技术特别是分子标记技术的的飞速发展，为蔬菜作物抗病基因的克隆奠定了基础。
- These results would be useful for interest gene cloning and marker-assisted selection for genetic improvement in Chinese jujube. 枣树高密度遗传连锁图谱的绘制和性状QTL定位为挖掘枣树有益基因打下了基础,为枣树分子遗传育种提供了依据。
- Dominating methods in wheat gene cloning include homology-based cloning, map-based cloning, suppression subtractive hybridization, DDRT-PCR etc. 目前，小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
- This paper summarized the progress of category and strategy of R gene cloning and the application of R gene cloned in Arabidopsis. 迄今为止，在拟南芥中已经克隆了十几个抗病基因。对拟南芥抗病基因的种类和特性、克隆策略以及克隆抗病基因的应用前景进行了综述。
- This review introduced the structure 、biologic activities and their mechanism of SAK, SAK’s gene cloning and expression research concerned. 本文从SAK的结构、生物学特性及机理、基因克隆及表达方面的研究进展等方面进行了综述。
