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- To construct fusion gene clone vector:pBluescript SK-CEA signal peptide-EK cutting peptide gene-HBD2 人CEA信号肽-EK位点-HBD2融合基因的构建
- These are inserted into a cloning vector. 它们被插入克隆载体。
- Objective To clone toxin_coregulated pilus subunit A (tcpA) gene of vibrio cholerae O139 strain XJ93006 and construct the recombinant cloning vector of pNEB 193_tcpA. 目的 以新疆分离 0 13 9霍乱弧菌XJ93 0 0 6株的DNA为模板 ,自行设计引物克隆毒素协同调节菌毛亚单位A(tcpA)基因 (包括侧序列 )并构建重组载体。
- In GENETIC ENGNEERING, nonviral DNA can be inserted into a phage, which is then used as a cloning vector. 在基因工程中,没有病毒的DNA可以被插入到噬菌体中,用作克隆载体。
- ACC Oxidase Gene Cloning and Sequence Analysis of Citrus aurantium L. 酸橙ACC氧化酶基因片段克隆及序列分析。
- An outline of the procedure for introducing a piece of foreign DNA into a cloning vector is shown in Figure 1. 3. 图1.;3列出了引出一段外源DNA进入克隆载体的大概步骤。
- This feature is favorable for gene cloning using transposon tagging strategy. 这个特性有利于转座因子作为分子标签克隆异源植物中的结构基因。
- Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in GNETIC ENGINEERING. 在许多双子叶植物中质粒在寄主细胞中是独立的复制单元,所以可以在基因工程中用作克隆载体。
- Gene cloning provides a means of purifying and propagating specific DNA segments. 基因克隆化提供了一种纯化和扩增特定DNA片段的方法。
- ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis. 通过引物设计、PCR、基因克隆、测序,获得了10种鸢尾属植物和1个外类群种的ITS序列。
- This paper summarizes and illustrates the strategy of how to do EST analysis and gene cloning through bioinformatics. 本文通过实例综述了怎样运用生物信息资源进行EST筛选及其新基因克隆的策略。
- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- Abstract Development of biotechnique, especially molecular marker technique, lays the foundation for R gene cloning. 摘要 分子生物学技术特别是分子标记技术的的飞速发展,为蔬菜作物抗病基因的克隆奠定了基础。
- Inducible prokaryotic expression vector pET-Nus-STK11(with Nus fusion tag) was constructed with pET-44a(+) and the cDNA of STK11 gene cloned in our lab. 利用本室克隆的人STK11 cDNA和原核表达载体pET-44a(+)构建带有Nus融合标签的诱导型表达载体pET-Nus-STK11,在不同的大肠杆菌宿主中诱导表达。
- These results would be useful for interest gene cloning and marker-assisted selection for genetic improvement in Chinese jujube. 枣树高密度遗传连锁图谱的绘制和性状QTL定位为挖掘枣树有益基因打下了基础,为枣树分子遗传育种提供了依据。
- Dominating methods in wheat gene cloning include homology-based cloning, map-based cloning, suppression subtractive hybridization, DDRT-PCR etc. 目前,小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。
- This review introduced the structure 、biologic activities and their mechanism of SAK, SAK’s gene cloning and expression research concerned. 本文从SAK的结构、生物学特性及机理、基因克隆及表达方面的研究进展等方面进行了综述。
- Here this article reviews the properties, gene cloning, structure and function of abrin, and its application in targeted cancer therapy. 本文就其性质、基因克隆、结构与功能,以及在肿瘤导向治疗方面的应用作一简要的综述。
- The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion. 将扩增的连接产物克隆入T载体,挑选阳性克隆并进行酶切鉴定,酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。
- Conclusions:E7 protein of HPV16 (HB strain) could be prepared by gene clone technique.HB-E7 protein displayed antigenicity of standard E7 protein. 结论:HPV16湖北株E7蛋白可通过基因克隆方法制备,并保留了标准E7蛋白的抗原性。