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- The quality of DNA by different methods of genome DNA extraction was different. 采用不同方法提取的3种松科植物叶片基因组DNA的质量差异较大。
- Methods:The genome DNA was distilled from Tetrandra Root,and differentiate it by RAPD(the random amplification polymorphism DNA). 方法:从独活药材中提取基因组DNA,运用RAPD(随机扩增多态性DNA)技术对其进行鉴别。
- Analyzed the genome DNA of high-protein wheat of T4 and CK by 34 random primers using RAPD. 选用34个随机引物对T4代高蛋白小麦和对照的基因组DNA进行分析。
- Extract the genomic DNA of a BLAB/c mice . 用酚氯仿异戊醇抽提blab/c鼠全基因组DNA;
- And the improved method for SDS was better than the one for CTAB in the quality of the genome DNA extracted from Cymbidium. 比较CTAB法和SDS法,改良的SDS法提取的春兰DNA质量与得率比改良的CTAB法相对要好,但就花药而言,还是改良的CTAB法(采用进口CTAB)较好,去除蛋白质相对干净。
- The thesis mainly reported that in the three methods used to extract genome DNA from S8 Aspergillus niger mycelium, the best was modified Vitagine Kit. 本文主要报道了用 3种不同方法从S8黑曲霉菌丝体中提取基因组DNA ,最适方法是经改进的Vitagene试剂盒法。
- Methods:The result of electrophoresis,purity and enzyme digesting of genome DNA of three kind of Pinaceae were compared. 方法:分别比较了用传统的CTAB法、SDS法以及3种改良的CTAB法提取的上述3种松科植物叶片基因组DNA的电泳、纯度和酶切效果。
- With the improved CTAB protocol, high quality genome DNA of the Lespedeza populations were obtained for RAPD amplification. 以改进的CTAB法获得了较高质量的胡枝子属植物基因组DNA,可以很好地用于RAPD扩增研究。
- Two RAPD fragments of Siniperca chuatsi virus (SCV) genome DNA recovered from agrose gel were inserted into plasmid pUC19 (called SCVE369 and SCVE450). 从RAPD扩增的鳜鱼病毒 (SCV)核酸电泳带中回收了二个片断 ,克隆子pUC19质粒 (称为SCVE36 9和SCVE45 0 )。
- Methods:The genome DNA was distilled from Dalbergia odorifera T.chen,and differentiate it by RAPD(the randomamplification polymorphism DNA). 方法:从降香药材中提取基因组DNA,运用RAPD(随机扩增多态性DNA)技术对其进行鉴别。
- This method is not only suitable to the arachnids, but also has certain model function to other arthropod genome DNA extraction. 盐析法不仅适用蜘蛛类,对其他节肢动物基因组DNA提取也有一定的借鉴作用。
- Objective To make genotyping for Chinese type 2 diabetes mellitus(NIDDM) pedigreed genome DNA and localizing the loci related genes with NIDDM. 目的对中国人2型糖尿病即非胰岛素依赖型糖尿病(NIDDM)家系基因组DNA进行基因分型及基因定位。
- Genome DNA in the fluid supernatant was conventionally extracted by proteinase K digestion followed by phenol/chloroform extraction. 胸/腹水上清基因组DNA的抽提采用常规蛋白酶K消化、酚/氯仿抽提法。
- And results attained were as followings:(1) A convenient and safe method on extracting the genome DNA from sweet orange, suitable for ISSR-PCR, was set. 获得了一种省时、简便的适用于ISSR-PCR扩增的甜橙基因组DNA提取的方法。
- Genomic DNA Extraction from the Polygonum multiflorum Thunb. 中药何首乌总基因组DNA的提取。
- The construct pGAPZB-Ds64PHR is transformed into Pichia pastor is and integrated into the genome DNA of Pichia pastoris. As above, the molecular biological test is also performed. 将表达载体pGAPZB-Ds64PHR重组到Pichia pastoris基因组DNA中,叨川大学硕士论文构建GSI 15一Ds“尸HR工程菌并进行了分子生物学验证。
- Method Genomic DNA was extracted by improved CTAB method. 方法采用改良CTAB法从罂粟叶片中提取DNA;
- Human genomic DNA of ALR consists of 3 exons and 2 introns. 克隆了人ALR基因组DNA全序列。
- Isolation of Genomic DNA from Cinnamomum cassia Presl. 中药肉桂基因组DNA的提取。
- The genome DNA of tobacco was extracted from the transgenic tobacco. The PCR, PCR-Southern and Southern results confirmed that the tha gene was integrated into the tobacco genome. 提取转基因烟草基因组总DNA,分别用PCR、PCR-Southern和Southern杂交检测转基因植株,结果显示thaumatin cDNA基因已整合到转基因烟草中。