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Hammerhead ribozyme can cleave the target NHE 1 mRNA specifically, reduce the expression of NHE 1 mRNA and induce intracellular acidosis. 所设计的锤头状核酶可对NHE 1mRNA进行特异性切割 ,减少其表达 ,使其活性下降 ,从而诱导细胞酸化。
Objective To detect the cleavage activity of Giardia canis virus (GCV) transfer vector-mediated hammerhead ribozyme for KRR1 in vitro transcript. 目的检测犬贾第虫病毒介导的锤头状核酶对犬贾第虫滋养体核仁功能性蛋白KRR1基因体外转录体切割效率。
Objective To study the transcription effects and cleavage activities of rat caspase-3 specific hammerhead ribozyme (Rz107) in both cell-free conditions and BRL-3A cells. 目的体外研究针对大鼠凋亡基因Caspase 3设计的核酶 (Rz10 7)的体外转录 ,切割及活性鉴定及其在大鼠肝细胞系BRL 3A细胞中的切割活性。
Aim: To explore the effects of sodium hydrogen exchanger(NHE 1) specific hammerhead ribozyme on the expression and activity of NHE 1 and pHi in pulmonary artery smooth muscle cells (PASMCs) in rats, and its role in PASMCs proliferation. 目的 :探讨锤头状核酶对大鼠肺动脉平滑肌细胞Na+ /H+ 交换器 1 (NHE 1 )表达和活性、pHi的影响 ,及其与细胞增殖的关系。
So we established three effective hammerhead ribozyme vector systems targeting +8, +36 and +71 of the VEGF mRNA, among which, the ribozyme targeting +36 site was the most efficient in all evaluations. 综上，我们构建的分别针对VEGF121 +8, +36,和+71位点锤头状核酶可有效地抑制VEGF的RNA水平，针对+36位设计的锤头状核酶抑制效果最好。
Keywords Dengue virus;Hammerhead ribozyme; 登革病毒;锤头状核酶;
INHIBITION OF TXS mRNA IN ECV304 CELL LINE BY HAMMERHEAD RIBOZYME 核酶抑制ECV304细胞TXS mrna的表达
Effect of Telomerase Hammerhead Ribozyme on Cervical Carcinoma Cell 端粒酶特异性核酶抑制宫颈癌细胞的作用
Keywords GCV;Transfer Vector;KRR1 protein;Hammerhead ribozyme; 犬贾第虫病毒;转染载体;KRR1蛋白;锤头状核酶;
Keywords HOGG1 Hammerhead ribozyme Drug sensitivity; 锤头状核酶;药物敏感性;
Substrates of the hammerhead ribozyme shift the internal equilibrium 锤头核酶的分子生物学机制
Keywords Giardia canis virus;Transfer vector;Hammerhead ribozyme;KRR1 protein; 犬贾第虫病毒;转染载体;锤头状核酶;KRR1蛋白;
Constructing the Hammerhead Ribozyme Gene's Eukaryon Express Vectors for hTR Sequence of Gallbladder Carcinoma 胆囊癌hTR锤头状核酶基因真核表达载体的构建
Effect of hammerhead ribozyme on NHE-1 activity in pulmonary artery smooth muscle cells of rats in vitro 锤头状核酶对大鼠肺动脉平滑肌NHE-1活性的影响
Reversal of multi-drug resistance of bladder cancer cell line with hammerhead ribozyme 核酶对膀胱癌细胞系多药耐药性的逆转研究
Constructing the Hammerhead Ribozyme Gene's Eukaryon Express Vectors for hTR Sequence of Gallbladder Carcin oma 胆囊癌hTR锤头状核酶基因真核表达载体的构建
Activity identification of anti-caspase-3 mRNA hammerhead ribozyme in both cell-free condition and BRL-3A cells 抗Caspase-3核酶的体外及BRL3A细胞内活性鉴定(英文)
Keywords Schistosoma japonicum（Chinese strain）;eggshell protein gene 2A;Hammerhead Ribozyme;ovulation development; 日本血吸虫（中国大陆株）;卵壳蛋白基因2A;锤头状核酶;虫卵发育;
Keywords Giardia lamblia;Pyruvate phosphate dikinase;Hammerhead ribozyme;Giardia canis virus; 蓝氏贾第鞭毛虫;丙酮酸磷酸双激酶;锤头状核酶;贾第虫病毒;
Keywords Hammerhead ribozyme;Androgen receptor;Gene expression;Carcinoma;prostate; 关键词核酶;雄激素受体;基因表达;癌;前列腺;