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- Methods We used heteroduplex mobility analysis (HMA) and sequencing to detect mixed infection. 方法使用异双体动态分析法和测序检测混合感染。
- Objective To compare the effectiveness of heteroduplex, SSCP and heteroduplex-SSCP analyses in mutational screening. 目的比较异源双链、单链构像多态(SSCP)、异源双链-SSCP法筛查突变的效率，寻找简便有效的基因突变筛查方法。
- The PCR products were then analyzed by heteroduplex, SSCP and heteroduplex-SSCP analyses separately to testify the effectiveness of each method. 方法应用聚合酶链反应(PCR)扩增23个已知的杂合子突变，分别以异源双链、SSCP、异源双链-SSCP法分析突变，对比各方法对突变的检出情况。
- Results Of the 23 mutations, 22 were detected by heteroduplex analysis (96%), 20 by SSCP analysis (87%) and 23 by heteroduplex-SSCP analysis (100%). 结果23个杂合子突变中，异源双链法、SSCP法与异源双链-SSCP法检出突变分别为22个(96%25)、20个(87%25)和23个(100%25)。
- Denaturing high- performance liquid chromatography was subsequently used to screen for the presence of heteroduplexes, and DNA sequencing was applied to these heteroduplexes. 我们接著以变性高效能液态层析法侦 测其中的异结合子，并以DNA定序的方法决定其DNA变异。
- Methods The microsatellite polymorphism of MICA exon 5 in 175 unrelated healthy individuals were investigated using PCR heteroduplex analysis. 方法用PCR-异源双链分析法,对175名正常无关个体的MICA基因第5外显子微卫星多态性的分布进行研究。
- Conclusion Heteroduplex-SSCP analysis is simple, economical and more effective than SSCP or heteroduplex analysis alone and it may be widely applied in mutational screening. 结论异源双链-SSCP法突变检出率高，经济、简便，值得在基因突变筛查中推广应用。
- Results from heteroduplex analysis are more stable and repeatable than those of SSCP and they may complement each other.Heteroduplex-SSCP analysis has the advantages of heteroduplex and SSCP analyses. 异源双链法结果比SSCP法结果稳定，重复性好，两种方法之间有一定互补性，异源双链-SSCP法兼有两种方法的特点。
- Methods By means of the modified heteroduplex mobility assay(HMA) and the routine DNA sequence analysis, the subtypes of 14 blood samples with HIV - 1 sera - positive were determined and analyzed and compared. 方法 采用改良的异源双链泳动法(HMA)与DNA序列分析法对14份HIV-1抗体阳性者样本进行亚型测定、分析和比较。
- heteroduplex chemical mismatch cleavage 异源双链化学错配切割
- heteroduplex migration retardation 异源双链的泳动延迟
- heteroduplex mobility assay(HMA) 异源双链泳动分析技术
- unidirectional heteroduplex repair model 单向异源双股修复模式
- Recombination: Heteroduplex and Mismatch Repair in vitro 重组:异源双链和体外错配修复
- COMPARISON OF HETERODUPLEX ANALYSIS AND SSCP IN GENE MUTATION SCREENING 异源双链分析和单链构象多态性分析在基因突变检测中的作用
- Rapid detection of genotypes of TT virus using a heteroduplex mobility assay 异源双链泳动分析法快速检测TT病毒基因型
- Application of Method to Eliminate the Heteroduplex Caused in the Course of PCR to Microbial Molecular Ecology 方法在微生物分子生态学研究中的一种应用
- Application of heteroduplex mobility assay in genetic subtyping on human immunodeficiency virus type 1 异源双链泳动分析法在人类免疫缺陷病毒1基因亚型分析中的应用
- Keywords HIV 1;genetic subtype;heteroduplex mobility assay （HMA）;molecular biology;molecular epidemiology; 人类免疫缺陷病毒1型;基因亚型;异源双链泳动分析法;分子生物学;分子流行病学;
- Keywords HIV 1 Genetic subtype;heteroduplex mobility assay（HMA）;molecular epidemiolog 人类免疫缺陷病毒1型;基因亚型;异源双链泳动分析法;分子流行病学;
