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- Utilizing this system, more than 50% hygromycin resistance selection efficiency was obtained with callus derived from young embryo (DREW, japonica ) and young panicle (D Shan B, indica ) as receptor, some regenerated plants were acquired. 依据该体系,以粳籼DREW 的幼愈伤组织和籼稻D汕B的幼穗愈伤组织为受体,经农杆菌AGL1/pCAMBIA1301转化后, 获得了超过50%25 的潮霉素抗性愈伤筛选率, 并获得再生植株。
- By the means of leave assay of hygromycin resistance, PCR amplification of a HPT fragment and Southern blot, it showed that RCOI and OsAOS fragments were successfully inserted into the rice genome. 利用离体叶片潮霉素快速鉴定、潮霉素PCR和Southern杂交等方法对转基因植株后代进行鉴定，证明了RCOI和OsAOS基因的片段正确插入水稻基因组；
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 标题： 图1 Rb反义基因表达载体结构示意图此表达载体Rb的转录方向与35S启动子的转录方向相反,并且含有GUS报道基因和抗潮霉素基因。
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系，对一些重要的影响银杏转化的因素，包括外植体类型，选择标记和筛选压，农杆菌工程菌株，共培养时间和恢复时间第一次进行了试验并优化了条件。
- Keywords Aspergillus fumigatus;Agrobacterium tumefaciens;Hygromycin resistance; 烟曲霉;根癌农杆菌;潮霉素抗性;
- Her resistance to the proposal has crumpled. 她对这个建议的抵触情绪已化为乌有了。
- Each of the bulbs has a resistance of120 ohms. 每一盏灯泡有120欧姆的电阻。
- Copper has less resistance than lead. 铜的电阻比铅的小。
- There has been a lot of resistance to this new law. 反对这项新法律的人很多。
- Our troops encountered only token resistance. 我们的部队仅受到表面上的抵抗。
- Stability of hygromycin resistant transformant of Colletotrichum lagenarium A, 潮霉素抗性转化子的稳定性
- Resistance stiffened even further last week. 上星期抵抗进一步加强了。
- The enemy gave up without resistance. 敌军没有抵抗就投降了。
- Every line in her face is the line of least resistance. 她脸上的每一根线条，都是阻小最小的线条。
- Only by determined resistance could their country be saved. 只有坚决抵抗，他们的国家才能得救。
- His resistance was worn down by constant nagging from his wife. 他的反抗被他妻子无休止的唠叨瓦解了。
- The strategy was designed to wear down the enemy's resistance. 这一策略旨在逐步削弱敌人的抵抗力。
- Having relatively high resistance to flow. 粘滞性的对流动有较高抵抗力的
- The firm has to overcome its resistance to new technology. 这家公司必须克服对采用新技术的阻力。
- hygromycin resistance 潮霉素抗性
