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It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
RESULTS: Most of the product didn t form inclusion body in cell. 结果:表达产物在细胞内大部分没有形成包含体。
The chicken inclusion body hepatitis virus could induce SPF chicken to synthesize TNF with the infection by mouth. 研究结果表明,鸡包涵体肝炎病毒经口腔感染SPF雏鸡可刺激机体产生TNF,在感染的不同日龄鸡的TNF活性出现一定的消长规律。
Objective To investigate the clinical and pathological features of inclusion body myositis (IBM). 摘要目的探讨包涵体肌炎的临床与病理特点。
Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法：对包涵体进行有效洗涤，采用疏水层析进行纯化。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body. 对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
Objective:To investigate the clinical and pathological features of inclusion body myositis(IBM). 目的探讨包涵体肌炎(IBM)的临床表现与病理特点。
The results showed that there was a silver-stainedDNA area(inclusion body,IB)in every cell infected with MCV. 结果显示：在被感染的皮肤表皮细胞内都有一个大小及构型不同的银染区（病毒工厂）。
Objective To choose the metal ions of immobilized metal affinity chromatography for separating the inclusion body of recombinant hepatitic E virus. 目的选择固相金属亲和层析柱的金属离子,以优化分离纯化重组戊型肝炎病毒包涵体的条件。
Several immune system cell types and processes have been recently identified in muscle in inclusion body myositis, dermatomyositis, and polymyositis. 近来，包涵体肌炎、皮肌炎、多发性肌炎中几种免疫细胞类型和免疫反应过程被认定。
The application of Hofmeister effect in recombinant product inclusion body denaturation and renaturation in biotechnology is discussed in particular. 目前重组蛋白生产过程中包含体的变性和复性问题为上述理论研究提供了广泛的应用空间。
The virus replicated in the intracytoplasm and formed inclusion bodies. 病毒在细胞浆内复制，可形成特征性包涵体；
In the multiplicating process, Lysosome constructs inclusion body of baculovirus by engulfing mitochondrias and pathogens, using the energy supplied by mitochondrias. 溶酶体在增殖过程中内吞濒临解体的线粒体和病原体，利用线粒体内嵴提供的能量，有规律地组装成杆状病毒的包涵体。
Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分离包涵体，确定1 M尿素洗涤包涵体为分离MP-GFP的最优条件。经Ni-NTA树脂纯化目标蛋白，SDS-PAGE电泳分析其纯度达到90%25以上。
The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%). VH和VL重组质粒分别转化到大肠肝菌BL21（DE3）中，IPTG诱导后，经SDS?PAGE电泳表明在12kD处有包含体蛋白表达，表达蛋白含量分别为28%25和35%25。
Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式，溶解和折叠后，以GST亲合层析色谱纯化。
With anti-CDV antigen assay, the ependymal cells with inclusion bodies showed an intensive positive reaction. 部分锥体细胞，神经核细胞和浦氏细胞变性、溶解或胞浆深染，胞核浓缩。
Swimming is the best way to tone up your body. 游泳是健体强身的最好方法。
It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. 它也包括关于包涵体，熔化蛋白质和结晶学的洗净的洗净的章。
The monolayer BEF with CPE was stained by HE, and the inclusion bodies were found in the enlarged cell nuclei. CPE表现为细胞肿大、变圆、脱落，HE染色可见到病变细胞内有核内包涵体。