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- inclusion body expression 包涵体表达
- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- RESULTS: Most of the product didn t form inclusion body in cell. 结果:表达产物在细胞内大部分没有形成包含体。
- The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%). VH和VL重组质粒分别转化到大肠肝菌BL21(DE3)中,IPTG诱导后,经SDS?PAGE电泳表明在12kD处有包含体蛋白表达,表达蛋白含量分别为28%25和35%25。
- The chicken inclusion body hepatitis virus could induce SPF chicken to synthesize TNF with the infection by mouth. 研究结果表明,鸡包涵体肝炎病毒经口腔感染SPF雏鸡可刺激机体产生TNF,在感染的不同日龄鸡的TNF活性出现一定的消长规律。
- Objective To investigate the clinical and pathological features of inclusion body myositis (IBM). 摘要目的探讨包涵体肌炎的临床与病理特点。
- The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies. 重组蛋白分泌到周质空间或胞外培养基中较之在胞内以包含体形式表达有许多优势。
- Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法:对包涵体进行有效洗涤,采用疏水层析进行纯化。
- The inclusion bodies were extracted by ultrasonic-breaking, and then the unique band was cut off from induced expression product separated by SDS-PAGE. 用超声波破碎细胞壁提取包涵体,SDS-PAGE结束后切下目的条带再用透析法复性浓缩,获得纯化的重组蛋白。
- Here's an exercise to help you use your body expressively. 这儿有一个练习帮助你使用你的身体的表现力。
- The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body. 对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
- Objective:To investigate the clinical and pathological features of inclusion body myositis(IBM). 目的探讨包涵体肌炎(IBM)的临床表现与病理特点。
- The results showed that there was a silver-stainedDNA area(inclusion body,IB)in every cell infected with MCV. 结果显示:在被感染的皮肤表皮细胞内都有一个大小及构型不同的银染区(病毒工厂)。
- Objective To choose the metal ions of immobilized metal affinity chromatography for separating the inclusion body of recombinant hepatitic E virus. 目的选择固相金属亲和层析柱的金属离子,以优化分离纯化重组戊型肝炎病毒包涵体的条件。
- Sometimes when you play, you hit a note or chord, and your facial and body expressions contort to match the audio expression. 有时当你演奏时,你击出一个音符或和弦,你的面部和身体表情会扭曲以配合听觉的表达。
- Several immune system cell types and processes have been recently identified in muscle in inclusion body myositis, dermatomyositis, and polymyositis. 近来,包涵体肌炎、皮肌炎、多发性肌炎中几种免疫细胞类型和免疫反应过程被认定。
- The virus replicated in the intracytoplasm and formed inclusion bodies. 病毒在细胞浆内复制,可形成特征性包涵体;
- The application of Hofmeister effect in recombinant product inclusion body denaturation and renaturation in biotechnology is discussed in particular. 目前重组蛋白生产过程中包含体的变性和复性问题为上述理论研究提供了广泛的应用空间。
- Keywords rhIL 10;Fusion expression;Inclusion body; 人重组白细胞介素-10;融合表达;包涵体;
- In the multiplicating process, Lysosome constructs inclusion body of baculovirus by engulfing mitochondrias and pathogens, using the energy supplied by mitochondrias. 溶酶体在增殖过程中内吞濒临解体的线粒体和病原体,利用线粒体内嵴提供的能量,有规律地组装成杆状病毒的包涵体。