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- LCB1 gene was cloned into inducible expression vector pYES2 and transformed into Saccharomyces cerevisiae FY2 The plasmid was induced to express with galactose. 酿酒酵母 (Saccharomycescerevisiae)LCB1 (Longchainbase)基因被克隆到酵母诱导表达载体pYES2中 ,并转入到FY2中 ,用半乳糖诱导表达。
- inducible expression vector 可调控表达载体
- Inducible prokaryotic expression vector pET-Nus-STK11(with Nus fusion tag) was constructed with pET-44a(+) and the cDNA of STK11 gene cloned in our lab. 利用本室克隆的人STK11 cDNA和原核表达载体pET-44a(+)构建带有Nus融合标签的诱导型表达载体pET-Nus-STK11,在不同的大肠杆菌宿主中诱导表达。
- Constructing the fused expression vector of elp gene of Echinococcusmultilocularis Sichuan isolate and its induced expression in E. Coli 四川株多房棘球绦虫elp基因融合表达载体的构建及其诱导表达
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- The plant expression vector pC-pPro-2-TCS was constructed with TCS structure gene integrated into downstream of PAL inducible promoter carried by pPPG expression vector. TCS gene was controled by linked promoter-PAL. pC-pPro-2-TCS是在pPPG表达载体的PAL诱导型启动子下游嵌合入TCS基因,TCS基因在串联启动子PAL控制下;
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Inducible Expression of Translation Elongation Factor 1A Gene in Rice Seedlings in Response to Environmental Stresses ? 环境因子胁迫下水稻翻译延伸因子1A基因的诱导表达(英文)
- induced expression vector 诱导表达载体
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9,将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
- The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表达载体P13w4一cTB和pcWIJN分别转化感受态根瘤土壤杆菌,经过PcR筛选和酶切鉴定,获得根瘤土壤杆菌转化子。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 为了高效表达人表皮生长因子,我们采用了家蚕杆状病毒表达系统(Bombyx mori baculovirus expression vector system, BMBEVS)。
- The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 设计带特定酶切位点的特异引物,分别克隆了麦冬BADH和CMO的编码区片段,构建了植物表达载体pCU1303-BADH和pCU1303-CMO。