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- The stable isotope labeling technique provided an ideal method for accurately quantitating the low abundance proteins which play a key role in cells or tissues. 近几年来,定量蛋白质组的技术发展很快,稳定同位素标记技术的提出,为准确定量在细胞或组织体系中发挥重要功能的低丰度蛋白质提供了一个理想的方法。
- isotope labeling technique 核素标记技术
- isotope labelling technique 放射性核素标记技术
- In this paper, these novel protein isotope labeling methods and alternative protein expression systems are reviewed. 本文旨在对这些蛋白质标记表达新技术的方法及应用予以逐一介绍。
- The divergent axon collaterals from neurons in locus coeruleus have been in vestigated by means of fluorescent double labeling technique in 36 rats. 用荧光素双标记法研究了36只大鼠蓝斑向前额叶皮质、海马、丘脑、小脑和脊髓的分支投射。
- Time-resolved fluoroimmunoassay technology is a non-radioimmunity labeling technique having most perspective future because of its unique advantage since 1980s. 20世纪80年代出现的时间分辨荧光免疫分析技术,以其独特的优势成为最有发展前途的非放射免疫标记技术。
- Methods Elivision labeling technique was used to study expression of P63 Actin CK in 18 cases of breast carcinoma in situ and 24 cases of early invasive carcinoma. 方法:采用免疫组化Elivision法检测18例乳腺原位癌,24例乳腺早期浸润性癌中P63 Actin CK的表达。
- isotope labelling techniques 同位素标记技术
- Acetic anhydride stable isotope labeling 乙酸酐稳定同位素标记
- Neurochemistry,flow cytometer,fluorescence labeling technique and Western blotting were adopted to detect activities of Na+ K+ ATPase and Ca2+ ATPase, levels of intracellular calcium and free calmodulin (CaM),and the total CaM expression. 采用神经生化、流式细胞仪、荧光标记术及Westernblotting等方法,定量观测了实验大鼠海马Na+-K+-ATP酶与Ca2+-ATP酶活性,细胞内游离钙离子含量与钙调素(CaM)相对活性平均通道荧光,以及海马组织总CaM表达的动态变化规律。
- RAPD molecular labeling technique is used, which is analyzed the mtDNA polymorphism of the wheat sterile line and maintainer line of S-type, K-type, Ven-type and T-type by primer S22 screen out steady molecular labeling of the mtDNA with these CMS. 采用RAPD分子标记技术,利用引物S22 对S 型、K 型、Ven型及T 型不育系及保持系mtDNA 进行了多态性分析,筛选出稳定的并与几种细胞质雄性不育相关mtDNA的分子标记。
- Methods: The morphology of HSF was observed under an electron microscope. The cell cycle was analyzed with flow cytometry. 3H TdR uptake was tested with isotope labeled method. 方法电镜观察细胞形态,流式细胞仪测定细胞周期,同位素放射测定3H胸腺嘧啶核苷(3HTdR)摄取量。
- The BBB score and Rivlin plate, SEP, retrograde and label technique of Horseradish Peroxidase, gross observation, histological and morphological analyses were taken as the observed indices. 运用脊髓神经功能(BBB评分和Rivlin斜板法)、体感诱发电位、辣根过氧化酶(HRP)示踪、大体观察、组织学及形态计量分析、超微结构变化作为观察指标进行实验观察。
- Methods The proliferation assay,NK cell killing activity assay and immunologic fluorescence double label technique were used to study four sear effect on T cell immunologic function. 方法 经增殖试验、NK杀伤活性试验及免疫荧光双标记方法研究了 4种血清对T细胞免疫功能的影响。
- Methods GABA receptor counts of peripheral branch of PTN (10 pairs painful infrior alveolar neuralgia and painless Lingual never) were measured by radioimmuoassay and isotope Labelling method. 方法通过同位素标记放射免疫法测定10对疼痛的下牙槽神经与无痛的舌神经末梢支GABA受体含量。
- Using HRP labeling techniques, we have demonstrated that there was a direct projection from anterior cingulate cortex to ventrobasal nucleus of the thalamus in the rat brain. 用HRP逆行示踪法证实了大鼠扣带回前部丘脑腹侧基底核投射通路。
- To study the use of nonradioactive isotope labeled substracts ~15N-Gly and ~13C-Palmitic Acid as tracers in measuring the adaptive absorptive function in a child having undergone total small bowel resection. 研究应用稳定同位素~（15）N－甘氨酸和~（13）C－棕榈酸示踪测定全小肠切除术后患儿的肠吸收功能。
- The metabolic kinetics of branched chain amino acids (BCAA) and aromatic amino acids (AAA) in normal subjects and patients with severe viral hepatitis as studied with 4 stable isotope labeled tracers. 作者用4种稳定性核素标记的支链氨基酸和芳香氨基酸对正常人与重型病毒性肝炎(重型肝炎)患者进行示踪动力学的研究。
- The basic principle,procedure and examples of the dansyl chloride fluorescence labelling technique are described. 介绍了用丹磺酰氯荧光标记法检测表皮角质层更新时间的基本原理、方法步骤和应用实例。
- TdT mediated nick-end labeling technique TdT介导槽口端标志法
