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- Serum can promote cultured cattle lens epithelial cells to adhere and proliferate. :血清可促进培养的牛晶状体上皮细胞贴壁和增殖
- Objective To study the effect of EMP on expression of P 21WAF1 in lens epithelial cells of dogs. 目的 观察电磁脉冲 (Electromagneticpulse ,EMP)对犬晶状体上皮细胞P2 1WAF1表达水平的影响。
- FACS and AO/EB stain were used to analysis the apoptosis of lens epithelial cells induced by H202. 流式细胞术、Ao/EB染色法分析过氧化氢诱导晶体上皮细胞凋亡作用。
- The human lens epithelial cells SRA01/04 were cultured in DMEM medium with 15% FBS, 37t ,5% C02. 将人晶体上皮细胞系SRA01/04细胞复苏后使用DMEM培养液,15%25优等胎牛血清,37℃,5%25二氧化碳进行培养,待细胞生长至一定数目,将其分为三组:实验组,空载体组,对照组。
- Objective: To observe the expression of epidermal growth factor (EGF) in lens epithelial cells(LECs) of human fetus. 目的:观察表皮生长因子(EGF)在人胎儿晶状体上皮细胞的表达。
- Methods: Using the developed procedure to culture rabbit lens epithelial cells, and the cultured cells were examined morphologically. 方法:利用改良消化法对兔晶体上皮细胞进原代培养,对培养的细胞进行形态学观察。
- The opacification is mainly due to the proliferation and migration of postoperative remnants of lens epithelial cells on the posterior lens capsule. 残留晶状体上皮细胞在后囊的增殖、移行、纤维化生是后囊混浊形成的重要因素。
- PCO is caused by the lens epithelial cells retained in the capsular bag following surgery, which proliferate, migrate and transform to myofibroblasts. 术后晶状体囊残留的晶状体上皮细胞的增殖、迁移、纤维化生是形成后发性白内障的主要原因。
- Objective To establish an effective method for culture of senile cataractous lens epithelial cells(LEC) and observe the growth of LEC in vitro. 目的观察老年性白内障晶状体上皮细胞体外培养的生长特点,为研究老年性白内障及术后后囊浑浊的发生机制及防治奠定基础。
- Object iv e To study the possibility of prevention of posterior capsular opacification(PC O)by transfer of exogenous gene into lens epithelial cells. 目的观察目的基因在晶状体上皮细胞的表达情况,以探讨基因疗法用于后发性白内障的可行性。
- Current interest in the prevention of PCO has centred around pharmacological methods to remove or destroy lens epithelial cells. 目前防治后发障的研究主要集中在药物除去或破坏残留晶状体上皮细胞。
- Conclusion The expression of MMP-2、MMP-9、TIMP-2、TIMP-1 on lens epithelial cells of age-related cataract is positive, and those of transparent lens are negative. 结论:老年性白内障患者晶状体上皮细胞阳性表达MMP-2、MMP-9、TIMP-2、TIMP-1,表达产物定位于细胞质。而四者在透明晶状体上皮细胞几乎不表达。
- Integrin is an important adhesion molecule that mediates cell-cell and cell-extracellar matrix adhesion and participates in the adhesion and migration of lens epithelial cells. 摘要整合素是介导细胞与细胞间及细胞与细胞外基质相互作用的重要黏附分子,其可参与晶状体上皮细胞的黏附和移行。
- WT5”BZ]To observe the differences between bovine lens epithelial cells before and after being frozen with transmission,scanning electron microscope and Flow Cytometer. 目的:本实验采用透射电镜和扫描电镜观察及流式细胞仪技术,比较了晶体上皮细胞冻存前后的变化,分析了晶体上皮细胞冻存的可行性。
- Objective To observe the infrastructure of lens epithelial cell in senile calraracts, and study the relation between senile cataract and lens epithelial cell apoplosis. 目的研究老年性白内障晶状体上皮细胞的超微结构,探讨老年性白内障与晶状体上皮细胞凋亡关系。
- Pharmacological experiments of Chinese herbal medicine were focused on antioxidant effect and inhibiting aldose reductase and decreasing apoptosis of lens epithelial cell. 中药制剂的实验药理研究主要集中在抗氧化作用、抑制醛糖还原酶作用和抑制晶状体上皮细胞凋亡方面。
- Conclusion Utilizing the designated primers combined with the 58.6℃ as anneal temperature, the amplified desired 302bp product , clear and consistent, was a prefer internal reference in quantitative mRNA analysis of cultured lens epithelial cells. 采用本实验中所设计的引物联合58.;6℃退火温度等实验条件所扩增的长度为302bp片段,清晰稳定,是晶状体上皮细胞mrna定量分析中良好的内部参照标准。
- Conclusion: Trypan-blue and alizarin red vital staining refer to lens epithelial cells, which was a practical methord to observe the morphology, density, area and the cellula vis vitalis. 结论:台盼蓝-茜素红活性染色可用于观察晶状体上皮细胞的形态、密度、面积以及细胞的活力,是研究晶状体上皮细胞的一种良好而实用的方法。
- The successful cloning and expression of DT389-hbFGF immunotoxin lay a foundation for accelerating lens epithelial cells apoptosis and the targeting therapy toward posterior capsule opacification. DT389-hbFGF免疫毒素克隆表达的成功为药物促晶状体上皮细胞的凋亡、抑制后发障的研究奠定了物质基础。
- Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed. 方法用绿色荧光蛋白基因作为报告基因,以逆转录病毒载体携之转染晶状体上皮细胞,观察GFP在晶状体上皮细胞的表达情况。