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- Mitochondrial linear plasmid DNAs are larger, and they contain terminal inverted repeats ,proteins covalently bound at 5' terminus,and longer ORFs. 线状线粒体DNA质粒的特征是分子较大，末端有重复序列，5'端与蛋白质共价结合，有较长的ORF。
- Methods The CHO/dhfr- cells were transfected with linearized plasmids of pMM-CTLA-4-IgG4/WG and pMMGR. The clones of CHO/pCTLA-4+/pMMGR+ were in suspension cultured in serum-free culture media. 方法将线性化的pMM-CTLA-4-IgG4/WG和pMMGR转染CHO/DG44细胞,对阳性克隆进行无血清悬浮培养,Western blot检测细胞培养液中CTLA-4/Ig融合蛋白的表达,筛选出高效稳定表达CTLA-4/Ig融合蛋白的CHO细胞;
- They harbor indigenously circular as well as linear plasmids,a useful source for developing genetic tool for studying of their unique physiology and metabolism. 在许多稀有放线菌中发现了环型与线型质粒,由质粒发展起来的遗传操作系统可以为研究稀有放线菌的生理及代谢提供强有力的工具。
- linearized plasmid 线型化质粒
- biotinylated linear plasmid 生物素化的线性质粒
- biotinylated linear plasmid DNA 生物素化线性质粒DNA
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- linear plasmid 线性质粒
- A specified arrangement used in ordering. An order need not be linear. 一种在排序中使用的特定排列。排列的次序不必是线性的。
- Second, non-linear factors should be linearized. 其次,对非线性因素的线性化;
- The ordinate scale is linear in decibels. 纵坐标比例尺以分贝为单位是线性的。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Here are linear measures in millimeters. 这是毫米方式的线性量度。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- A linear differential transformer has three coils. 一个直线差动变压器有三个线圈。
- The PNL-BDNF-IRES2-EGFP plasmid was identified to be correct. 构建的慢病毒载体质粒PNL-BDNF-IRES2-EGFP经Sal I和BamH I双酶切鉴定正确,三质粒共转染293T细胞后荧光激发可见大量绿色荧光。
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
