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- Study of detecting BCR/ABL fusion gene by using nest RT PCR 应用筑巢式RT-PCR检测BCR-ABL融合基因的探讨
- Five mites from Ag positive mice, 50, 10 mites from Ag negative mice, 10, 5 free mites and 100 mg mouse lung of Ag positive mice were positive by nested RT PCR instead of negative by RT PCR. HFRSV抗原阳性鼠体恙螨5只组，HFRSV抗原阴性鼠体恙螨50只和10只组，游离螨10只和5只组，鼠肺HFRSV抗原阳性100mg组均未见有明显扩增带。
- Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced. 方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- RT PCR for EWS FLI 1 is sensitive enough to detect minimal residual Ewing's sarcoma cells in the bone marrow. 以EWS FLI 1融合基因为标志的反向PCR测定骨髓Ewing肉瘤细胞的灵敏度为 10 -6,它是测定骨髓Ewing肉瘤细胞高度敏感的方法 ;
- The positive rate of MDR1 mRNA was 70.3% with improved RT PCR in 37 among 49 cases of ovarian carcinomas. 有37 例卵巢癌用改进的RT?PCR 检测MDR1 基因m RNA 表达的阳性率为70?3 %25 。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT－PCR技术从人B淋巴瘤细胞系Raji中克隆到B7－1（CD80）cDNA，并经测序证实。
- Methods B7 expresion was studied by means of RT PCR and immunohistochemistry (IH) in 45 tumors of the urinary system. 方法应用逆转录?聚合酶链反应（RT?PCR）和免疫组织化学方法，检测45例泌尿系肿瘤B7基因在mRNA和蛋白水平的表达情况。
- Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR). 方法采用逆转录聚合酶链反应（RT－PCR）检测84例静脉毒瘾者血浆标本。
- Methods RT PCR was used to explore IL 1Ra mRNA expression in middle cerebral artery occlusion (MCAO) rat model. 方法 采用线栓法制备SD大鼠局灶性大脑中动脉阻塞 (MCAO)动物模型 ,应用RT PCR方法观察脑缺血再灌注对大鼠IL 1RamRNA表达的影响。
- After the DC were transfected with pCA13/EWS FLI1,the EWS FLI1 mRNA was easily detected by RT PCR. pCA13/EWSFLI1转染DC后,RTPCR检测DC中有EWSFLI1mRNA的表达。
- Methods: The expression of B7 in 22 cases of renal cell carcinoma was studied by RT PCR and immunochemical methods. 方法：本文应用RT?PCR和免疫组织化学技术检测了22例肾细胞癌组织B7的表达。
- It can be measured the expression of c jun and c fos by using the methods of RT PCR and agarose gel electrophoresis. 应用RT PCR和琼脂糖凝胶电泳方法测定c jun和c fos基因的表达。
- Total RNA was extracted from PC12 cells. The TRX cDNA obtained by RT PCR was cloned into PUC18vector. 从PC1 2细胞中提取总RNA ,经逆转录PCR(RT PCR)扩增出硫氧还蛋白cDNA并克隆到PUC1 8质粒上。
- Methods Using HE stains,Niles stains,transmission electron microscope,immunohistochemistry and RT PCR technique etc. 方法采用HE染色、尼氏染色、透射电镜、免疫组化及RT-PCR技术。
- Methods The expressions of AQP1-9 in various organs of male reproductive tract of mouse was determined using RT PCR. 方法 :应用 RT- PCR技术检测 AQP1 - 9在雄性小鼠生殖系统不同器官的表达。
