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- Keywords cationic Liposome;noncoding Plasmid DNA;colon cancer;gene thera; 阳离子脂质体;非编码质粒DNA;结肠癌;基因治疗;
- noncoding Plasmid DNA 非编码质粒DNA
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- Objective To develop a procedure for large-scale purification of plasmid DNA. 摘要目的建立适合大规模纯化质粒DNA的工艺。
- P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III. 扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
- This arti cle reviews the structure of plasmid DNA, especially the structure of its Assist ant units and effects. 本文阐述了基因免疫中质粒DNA中的结构组成特别是其佐剂单位及其作用。
- Plasmid DNA and calf-DNA are irradiate d by laser with the samestrengty and different irradiation period. 激光作用质粒DNA和小牛胸腺DNA产生损伤效应，导致DNA结构变化。
- The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. 实验室中常用的质粒提取方法是碱裂解法。
- Switch on the vacuum to draw the solution through the column (The plasmid DNA binds to the column material). 开启真空装置使液体通过管柱(质体DNA会与管柱物质结合)。
- Ion exchange HPLC is an alternative that yields milligram quantities of purified plasmid DNA within an hour. 离子交换HPLC作为替代方法可以在一小时内生产毫克量的纯化的质粒DNA。
- Routine alkaline lysis miniprep of plasmid DNA was modified,and LiCl was specially used. 对碱裂质粒小量法进行了改进，并且在提取过程中增加了LiCl处理。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- Methods Plasmid DNA was extracted by the procedure of Kado and Liu,and Pgm was detected by incubating isolates on Congo red media at28oC for7days. 方法质粒运用Kado改良法进行提取; Pgm利用刚果红培养基置28℃培养7天观察。
- A variety of approaches have been taken to augment and modulate the immunogenicity and efficacy of plasmid DNA vaccines. 核酸疫苗虽然在小动物实验中取得成功,但对大动物、灵长类动物和人类的免疫原性却很低。
- Assuming that the plasmid DNA is endotoxin-free, mention how you think "danger" is signaled by DNA of bacterial or plasmid origin (5 points). 假设这个质体DNA不含内毒素，提出理由说明你认为"危险"讯息是源自细菌的DNA或是质体（5分）。
- The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA. 利用低功率HeNe激光辐照离体质粒DNA，分析了质粒DNA的损伤效应和lacZ基因的突变。
- Luciferase plasmid DNA and PEI were complexed to form slightly positive-charged nanoparticles, onto which FOL-PEG-PLL conjugate was surface coated. 首先用荧光素酶质粒DNA和PEI合成略带正电的纳米颗粒，再在其表面包裹FOL-PEG-PLL共轭化合物。
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA，直接转化大肠杆菌。
- The money will be used to build up a state-of-the-art cGMP level manufacturing plant for vaccine production and plasmid DNA contract manufacturing business. 国光生技公司在历届经理人稳健经营的领导下，本著以开发生产疫苗为职志，为维护人类健康而努力。
- Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used.Electron microscope photos were prepared and observed. 方法：应用琼脂糖凝胶电泳试验、电镜照片制备及观察、粘附试验及质粒消除试验。
