pBinAR-aPG and pBinAR-PG were transferred into Agribacturillus EHA105 respectively by freeze thawing method which could be used for further transformation.

• 把两个表达载体pBinAR-aPG和pBinAR-PG通过冻融法分别导入农杆菌EHA105感受态,为进一步遗传转化作准备。