该词条未找到_海词词典

您要查找的是不是：

Isolation and plant expression plasmid construction of 32-kDa secretary protein gene of mycobacterium bovis BCG strain D2 卡介苗D2株分泌蛋白基因植物表达载体的构建
plant expression plasmid 植物表达载体
A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter. 以pC1303质粒为基础,构建了均由35S启动子驱动的CMO基因和BADH基因的植物双基因表达载体pC35SC35SB1303。
In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
Obtaining of MCEMA(modified CEMA) and plant defensin AFP geneMCEMA (141bp) was amplified with pUCSPCEMA plasmid as template and P5, P4 as primers, and plant expression vector pEMCEMA was constructed. MCEMA和植物防御素基因的PCR合成以经测序验证的SPCEMA为模板，用P_5和P_4扩增得到了全长141bp的MCEMA，并构建了植物表达载体pEMCEMA。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed. 再以此为基础构建得到编码FasL和VEGF165的共表达质粒 pCI FasL IRES VEGF165 (简称pCI FIV )。
Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 将构建好的重组质粒pKK223.;3-RTA和pKK223
The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB . 将乙肝表面抗原基因(adw型)与细胞核载体PB、PBG以及叶绿体表达载体PLCTB进行定向克隆，经PCR和测序鉴定都获得了重组子，分别命名为PB-adw、PBG-adw、PLCTB-adw。
And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 设计带特定酶切位点的特异引物,分别克隆了麦冬BADH和CMO的编码区片段,构建了植物表达载体pCU1303-BADH和pCU1303-CMO。
Methods: 1.The KK gene was directional cloned into the pAAV-MCS. Thisrecombinant pAAV expression plasmid was called pAAV-KK. 方法:1.;将KK 基因定向克隆入腺相关病毒载体质粒pAAV-MCS 中构建成pAAV-KK。
To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通过基因重组技术体外构建真核表达质粒pcDNA3.;1(+)/GDF-5;并检测其在小鼠骨髓基质干细胞中的表达。
Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
Through the gene gun to transfer the AWTE CTB plant expression vector pBVG ny2 which containing NPTII and GUS gene into the soybean tissue. 把 AWTE- CTB融合基因构建到植物表达载体 p BVG- ny2上 ,通过基因枪导入法 ,转化大豆幼胚分生组织。
Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。